白藜芦醇对大鼠重症急性胰腺炎肠黏膜细胞的保护作用及其机制

目的 探讨白藜芦醇对实验性重症急性胰腺炎(SAP)大鼠肠黏膜细胞的保护作用及其可能的作用机制.方法 24只成年雄性SD大鼠随机分为假手术(SO)组、重症急性胰腺炎(SAP)组、白黎芦醇治疗(RES)组.SO组于开腹翻动十二指肠后关腹,SAP组大鼠以40g/L牛磺胆酸钠1 ml/kg经胰胆管逆行注射复制胰腺炎模型.RES组于胰腺炎模型复制成功后立即经阴茎背经脉注射白藜芦醇(10mg/kg).各组大鼠均于制模后6h采集标本.动态浊度法检测血清内毒素含量,TUNEL法检测肠黏膜上皮细胞凋亡率,RT-PCR检测凋亡调控基因Bax、bcl-2mRNA表达,激光共聚焦显微镜检测肠黏膜细胞线粒体膜电位变化.结果 RES组血清内毒素水平和肠黏膜细胞凋亡指数均低于SAP组(P＜0.01).RES组BaxmRNA表达低于SAP组(P＜0.01),bcl-2mRNA表达高于SAP组(P＜0.01).RES组线粒体膜电位高于SAP组(P＜0.01).结论 白藜芦醇可通过抑制肠黏膜上皮细胞的凋亡,维持肠黏膜屏障的完整性,从而防止SAP时细菌和内毒素的移位.

Protective effect of resveratrol on the intestinal mucosal cells in rats with severe acute pancreatitis and the mechanism

Objective To investigate the protective effect of resvertrol on the intestinal mucosal cells in rats with severe acute pancreatitis and explore the possible mechanism.Methods Twenty-four SD rats were randomly divided into the sham-operation(SO) group,severe acute pancreatitis(SAP) group and resveratrol-treated(RES) group.In the SO group,the pancreases were slightly flipped only.In the SAP and RES groups,SAP model was established by retrograde injection of 40 g/L sodium chrolate(1 ml/kg) through the pancreat...