缬沙坦对心力衰竭家兔心肌肌浆网钙调控相关蛋白基因的影响

目的探讨家兔慢性心力衰竭(心衰)时心肌肌浆网钙泵(SERCA2)、受磷蛋白(PLB)基因表达的改变及血管紧张素Ⅱ受体拮抗剂缬沙坦长期干预的意义。方法18只家兔随机分为3组,假手术组、心衰组和缬沙坦组,每组6只。通过超容量负荷联合压力负荷建立家兔心衰模型,术后7周观察左心室结构、血流动力学的变化及SERCA2、PLB基因的表达。结果与假手术组比较,心衰组左心室重量指数、心率、左心室舒张末压显著升高(P<0.05),左心室缩短率及LVEF明显降低(P<0.05);与心衰组比较,缬沙坦组左心室重量指数、心率、左心室舒张末压显著降低(P<0.05),左心室缩短率及左心室射血分数明显升高(P<0.05);心衰组SERCA2、PLBmRNA显著低于假手术组(P<0.05),缬沙坦组SERCA2、PLBmRNA显著高于心衰组(P<0.05)。结论缬沙坦长期干预心衰,能够改善心脏舒缩功能,可能与其上调肌浆网的钙调控蛋白SERCA2、PLB基因表达有关。

Effect of valsartan on sarcoplasmic reticulum Ca2+ handling correlative proteins gene in heart failure rabbit

Objective To investigate the expression of myocardial sarcoplasmic reticulum calcium handling correlative proteins(sarcoplasmic reticulum calcium adenosine triphosphatase,SERCA2,and phospholamban,PLB)gene in heart failure rabbit and the effects of valsartan on the prevention of chronic heart failure.Methods Eighteen rabbits were divided into three groups:6 rabbits with heart failure induced by volume plus pressure overload,6 sham operated rabbits and 6 rabbits treated with valsartan.Seven weeks later,left ventricular function,hemodynamic parameters,and SERCA2,PLB gene expression were observed.Results Compared with the sham operated rabbits,HR,LVMI and LVEDP in heart failure rabbits were significantly increased(P<0.05),while their left ventricular shorten fraction and ejection fraction were decreased(P<0.05).Compared with the heart failure rabbits,HR,LVMI and LVEDP in the valsartan treated rabbits were significantly decreased(P<0.05),while their left ventricular shorten fraction and ejection fraction were increased(P<0.05).SERCA2 and PLB gene expression in heart failure rabbits were remarkably lower than that in sham operated rabbits and valsartan treated rabbits(P<0.05).Conclusion Valsartan can improve cardiac function,probably owing to its upregulating gene expression of myocardial sarcoplasmic reticulum calcium handling correlative proteins(SERCA2 and PLB).