参附注射液对缺血再灌注肢体保护作用

目的： 探讨参附注射液（SF）对缺血再灌注肢体保护作用及机制。方法: 30只月龄相当的健康大鼠随机分为A、B、C、D、E 5组，A组为单纯缺血再灌注组（IR）， B组、C组分别于阻断血流前 30 min 股动脉注射参附注射液 10 mL/kg、20 mL/kg; D组和E组分别于再灌注后立即股动脉注射参附注射液 10 mL/kg、20 mL/kg。5组动物均在股动静脉阻断前后 1 h 及再灌注后 1 h、2 h 分别从股静脉采集血样，测定血浆中肌酸磷酸激酶（CPK）、谷草转氨酶（GOT）的含量以及测定血浆超氧化物歧化酶（SOD）、丙二醛（MDA）的含量。结果: 各治疗组中SOD活性明显高于IR组,MDA、CPK、 GOT水平则明显低于IR组。但B组和C组之间有明显差异。而B、C组与D、E组之间有明显差异。结论: 参附注射液对缺血再灌注肢体具有保护作用，但术前给药、剂量 20 mL/kg 效果更佳。

Protective effects of Shengfu injections on limbs in ischemia/reperfusion injury

AIM: To study the protective effects of Shengfu injections on the limbs in ischemia/reperfusion injury. METHODS: 30 healthy rats at the same age were divided into 5 groups randomly. Group A was the simple reperfusion of ischemic limbs. Group B and C were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg. Group D and E were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg right after reperfusions. Blood samples were collected from femoral veins 1 h before femoral vessels were blocked, 1 h and 2 h after reperfusions in all animals. The concentrations of the CPK, GOT, SOD and MDA in the blood samples were determined. RESULTS: MDA increased, SOD, CPK, GOT decreased markedly in the therapeutic groups.CONCLUSION: The Shengfu injections surely protected the limbs from ischemia/reperfusion injury, the better effects was observed when the drug was administered before operation at the dose of 20 mL/kg.