| Flk-1_(265-2493)与C3d3基因融合质粒诱导小鼠免疫应答的初步研究 |
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| 引用本文： | 梁培禾,张克勤,聂志林,许桂莲,李彦锋,叶锦,靳风烁. Flk-1_(265-2493)与C3d3基因融合质粒诱导小鼠免疫应答的初步研究[J]. 第三军医大学学报, 2009, 31(13) |
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| 作者姓名： | 梁培禾 张克勤 聂志林 许桂莲 李彦锋 叶锦 靳风烁 |
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| 作者单位： | 1. 大坪医院野战外科研究所泌尿外科,重庆,400042
2. 基础医学部全军免疫学研究所,重庆,400038 |
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| 摘 要： | 目的 对pSG.SS.Flk-1265-2493·C3d3.YL及pSG.SS.Flk-1265-2493·YL真核表达质粒的免疫效应进行初步研究.方法 构建pSG.SS.Flk-1265-2493·C3d3.YL及pSG.SS.Flk-1265-2493·YL真核表达质粒后,48只BALB/c小鼠随机均分为疫苗组、疫苗对照组、质粒对照组、空白对照组(12只/组),分别肌肉注射接种此2种质粒、pSG.SS.YL质粒和PBS.免疫后动物:①每组6只取脾脏分离细胞,MTT法测定经Flk-1蛋白刺激后的增殖效应,乳酸脱氢酶释放法测定对培养小鼠肺微血管内皮细胞的杀伤作用;②其余6只动物于不同时间点断尾取血分离血清,ELISA法测定抗Flk-1抗体水平的动态变化.结果 经Flk-1蛋白刺激,疫苗组小鼠脾淋巴细胞的增殖效应明显较强,刺激指数显著高于其他3组(P均＜0.01),对靶细胞(MVECs)的杀伤活性在不同效/靶比时均显著高于其他3组(P均＜0.01).疫苗组动物血清抗Flk-1抗体比对照疫苗组抗体阳性反应出现早2周,二者峰值效价均出现在5周(血清稀释度:1:3840 vs 1:60),前者12周时仍保持较高水平(1:960),后者10周时抗体阳性反应已消失.结论 C3d3的连接有效增强了Flk-1265-2493的免疫原性.
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| 关 键 词： | 血管新生 DNA疫苗 |
Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1_(265-2493) and three copies of C3d |
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| LIANG Pei-he,ZHANG Ke-qin,NIE Zhi-lin,XU Gui-lian,LI Yan-feng,YE Jin,JIN Feng-shuo. Immunological effect of a DNA vaccine encoding genetic fusion of Flk-1_(265-2493) and three copies of C3d[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(13) |
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| Authors: | LIANG Pei-he ZHANG Ke-qin NIE Zhi-lin XU Gui-lian LI Yan-feng YE Jin JIN Feng-shuo |
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| Affiliation: | LIANG Pei-he1,ZHANG Ke-qin1,NIE Zhi-lin1,XU Gui-lian2,LI Yan-feng1,YE Jin1,JIN Feng-shuo1(1Department of Urology,Daping Hospital,Institute of Surgery Research,Third Military Medical University,Chongqing 400042,2Institute of Immunology,College of Basic Medical Sciences,Chongqing 400038,China) |
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| Abstract: | Objective To evaluate the immunological effect of eukaryotic expression plasmids pSG.SS.Flk-1265-2493.C3d3.YL and pSG.SS.Flk-1265-2493.YL. Methods After construction of eukaryotic expression plasmids pSG.SS.Flk-1265-2493.C3d3.YL and pSG.SS.Flk-1265-2493.YL, BALB/c female mice (n=48) were separated into four groups (n=12 per group) and were inoculated by intramuscular injection of the two plasmids, pSG.SS.YL plasmid (plasmid control) and PBS (blank control), respectively. After inoculation, proliferation of ... |
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| Keywords: | Flk-1 C3d |
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