| HPLC同时测定山核桃外蒲壳中球松素和大黄酚含量 |
| |
| 引用本文： | 吴德玲,陈仕云,金传山,刘劲松,彭代银.HPLC同时测定山核桃外蒲壳中球松素和大黄酚含量[J].药物分析杂志,2012(2):249-251. |
| |
| 作者姓名： | 吴德玲 陈仕云 金传山 刘劲松 彭代银 |
| |
| 作者单位： | 安徽中医学院药学院;安徽省现代中药重点实验室;合肥学院化学与材料工程系;现代中药安徽省工程技术研究中心 |
| |
| 基金项目： | 安徽中医学院合作研究项目(2008HZ001) |
| |
| 摘 要： | 目的:建立同时测定山核桃外蒲壳中球松素和大黄酚含量的分析方法。方法:采用HPLC法,色谱柱为Shim-pack C18(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液(79∶21)为流动相,流速0.8 mL·min-1,检测波长286 nm,柱温25℃。结果:山核桃外蒲壳中球松素和大黄酚的线性范围分别为4.12～25.7μg·mL-1和1.01～13.4μg·mL-1,平均加样回收率(n=6)分别为98.8%和98.0%。结论:该方法准确可靠、结果稳定、重复性好,为评价山核桃外蒲壳的质量提供依据。
|
| 关 键 词： | 山核桃外蒲壳 球松素(乔松酮) 大黄酚 高效液相色谱法 |
HPLC simultaneous determination of pinostrobin and chrysophanol in hull of Carya cathayensis Sarg |
| |
| WU De-ling,CHEN Shi-yun,JIN Chuan-shan,LIU Jing-song,PENG Dai-yin.HPLC simultaneous determination of pinostrobin and chrysophanol in hull of Carya cathayensis Sarg[J].Chinese Journal of Pharmaceutical Analysis,2012(2):249-251. |
| |
| Authors: | WU De-ling CHEN Shi-yun JIN Chuan-shan LIU Jing-song PENG Dai-yin |
| |
| Institution: | 2,3(1.Department of Pharmacy,Anhui College of Traditional Chinese Medicine,Hefei 230031,China; 2.Anhui Key Laboratory of Traditional Chinese Medicine,Hefei 230031,China; 3.Anhui Engineering Research Center for Modern Chinese Materia Medica,Hefei 230031,China; 4.Department of Chemical and Materials Engineering,Hefei University,Hefei 230031,China) |
| |
| Abstract: | Objective:To establish a method for simultaneous determination of pinostrobin and chrysophanol in the hull of Carya cathayensis.Methods:The analysis was carried out on a Shim-pack C18(250 mm×4.6 mm,5 μm) column eluted with a mobile phase of methanol-water containing 0.1% phosphoric acid(79∶ 21);The flow rate was 0.8 mL·min-1 and column temperature was 25 ℃;The detection wavelength was set at 286 nm.Results:The calibration curves of pinostrobin and chrysophanol were linear in the ranges of 4.12-25.7 μg·mL-1 and 1.01-13.4 μg·mL-1,and the average recoveries(n=6) were 97.8% and 98.0%,respectively.Conclusion:The method is simple,accurate and sensitive,which can be used as a method of quantitative determination of pinostrobin and chrysophanol in the hull of Carya cathayensis. |
| |
| Keywords: | hull of Carya cathayensis Sarg pinostrobin chrysophanol HPLC |
| 本文献已被 CNKI 等数据库收录！ |
|
