细胞周期蛋白D1基因多态性与肝细胞癌遗传易感性的相关性

目的:探讨细胞周期蛋白D1(Cy-clinD1)基因A870G多态性与肝细胞癌(hepatocellular carcinoma,HCC)遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测225例HCC与428名对照的CyclinD1A870G基因型分布及差异。结果:全样本以及男性样本HCC与对照间的基因型分布差异均无统计学意义。但在女性,HCC组870G等位基因频率显著高于对照组(53.2%vs42.0%),P=0.012。与A/A纯合子相比,A/G杂合子的HCC风险增加至1.54倍(95%CI:0.79~3.01),P=0.202,而G/G纯合子的HCC风险显著增加至2.63倍(95%CI:1.20~5.78),P=0.016。结论:CyclinD1870G增加女性HCC发病风险,G/G基因型是女性患HCC的一个遗传易感因素。

Correlation of Cyclin D1 polymorphism with genetic susceptibility to hepatocellular carcinoma

OBJECTIVE:To evaluate the correlation of Cyclin D1 A870G polymorphism with genetic susceptibility to hepatocellular carcinoma (HCC) in a Chinese population. METHODS: Two hundred and twenty-five HCC cases and 428 controls were studied. The Cyclin D1 genotypes were determined by a PCR-based restriction fragment length polymorphism (RFLP) method. RESULTS: Overall, no correlation between HCC and the A870G genotypes was found when comparing all HCC cases to the controls or when comparing the male cases to the male controls. However, in the females, the Cyclin D1 870G allele was more frequently observed in the HCC group (53.2% vs 42.0%, P=0.012) than the control group. As compared with A/A homozygotes, A/G heterozygotes had a 1.54-fold increased risk (95%CI: 0.79-3.01, P=0.202), whereas G/G homozygotes had a 2.63-fold (95%CI: 1.20-5.78, P=0.016) increased risk for HCC. CONCLUSION: The G allele of the Cyclin D1 A870G polymorphism is correlated with the presence of HCC and G/G homozygote is potentially one of the genetic risk factors for HCC in females.