抗内毒素Fab'对严重烧伤早期炎症细胞因子的影响

目的 观察抗内毒素Fab'对严重烧伤早期肠源性内毒素血症小鼠肠道损伤的保护作用.方法 采用严重烧伤早期肠源性内毒素血症小鼠模型,分为烧伤组、治疗组及对照组,分别于6、12、24、48 h四个时相点测定血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-lβ、IL-10的浓度.结果 与正常对照组比较,烧伤后血清TNF-α、IL-1β、IL-10水平增高,差异有统计学意义(P<0.01);治疗组血清TNF-α、IL-1β、IL-10水平较烧伤组显著降低(P<0.01).病理检查结果提示治疗组较烧伤组肠黏膜损伤明显减轻.结论 抗内毒素Fab'能抑制内毒素所诱导的TNF-α、IL-1β产生,同时调节血清中的IL-10水平,减轻内毒素对机体的损害,从而起到对严重烧伤后肠源性脓毒症的防治作用.

Effects of Fab' against LPS on inflanunatory cytokines during the early stage of severe burns in mice

Objective To investigate the effect of the Fab' against LPS on intestinal mucosa during the early stage of severe bums in mice. Methods The gut-derived endotoximia mice model in the early stage of severe burn was created. The mice were randomly divided into three groups: burn group, control group and treatment group. At 6,12,24 and 48 h after burns ,levels of serum TNF-a,IL-1β and IL-10 were detenninated by enzyme linked immunosorbant assay. Results After burns,levels of serum TNF-α,IL-1 β and IL-10 in burn group were significantly higher than those in the control group (P<0.01). The levels of serum TNF-α,IL-β and IL-10 in treatment group were significantly lowere than in the burn group (P < 0.01 ). Pathological examinations revealed that the damage to the intestinal mucosa in treatment group was obviously alleviated as compared with bum group. Conclusion The Fab' against LPS can significantly decrease the levels of serum TNF-α,IL-1β and IL-10 during the early stage in severely burned gut-derived endotoximia mice, and inhibit injury by LPS,then prevent and cure burn sepsis.