异丙酚和异氟醚后处理对内毒素血症大鼠肺泡液体转运功能的影响

目的 建立内毒素血症大鼠模型,应用异丙酚和异氟醚,以肺组织湿干重比、肺泡液体清除率、肺水通道1为指标,观察肺泡液体转运功能的变化.方法 健康SD大鼠32只,随机分为4组.生理盐水组:尾静脉注射生理盐水;内毒素组、异丙酚组和异氟醚组均静注内毒素.1小时后.异丙酚组泵注异丙酚,异氟醚组持续异氟醚吸入.2小时后取标本,测定肺组织湿干重比、肺泡液体清除率:Western blot法检测水通道1蛋白.结果 各组肺组织湿/干比差异均尤统计学意义.异氟醚组肺泡液体清除率(0.054±0.007)较异丙酚组(0.065±0.006)高;异氟醚组水通道1为(0.53±0.06)较异丙酚组(0.60±0.07)高,均有显著差异(P＜0.05).结论 与异丙酚相比,异氟醚后处理增加内毒素血症大鼠肺泡液体清除率和水通道1蛋白表达.

The effect of posttreatment with isoflurane versus propofol on the function of alveolar fluid transference in endotoxemic rats

Objective To measure the wet-weight/dry-weight ratio,alveolar fluid clearance and aquaporin 1 in endotoxemic rats,observe if there is any change undergoing isoflurane or propofol.Methods Thirty two SD rats were randomized into four groups (n=8),NS group were administrated with NS.Propofol,isoflurane,endotoxin group were administrated with endotoxin.After 1 hour, Propofol group and isoflurane group were undergoing 2 hours with propofol or isoflurane respectively. Measure the wet-weight/dry-weight ratio,alveo...