| S100A13基因的高表达对HepG2细胞生物学性状影响的研究 |
| |
| 引用本文： | 刘应兰,杨靖,钟警,曹仁贤. S100A13基因的高表达对HepG2细胞生物学性状影响的研究[J]. 中国现代医学杂志, 2011, 21(16) |
| |
| 作者姓名： | 刘应兰 杨靖 钟警 曹仁贤 |
| |
| 作者单位： | 南华大学附属第一医院内分泌科,湖南衡阳,421001 |
| |
| 摘 要： | 目的 观察S100A13基因的高表达对HepG2细胞生物学性状的影响.方法 采用以绿色荧光蛋白(GFP)为报告基因的重组表达质粒PcDNA3.1/NT-GFP-S100A13,经脂质体介导转染肝癌细胞系(HepG2);荧光显微镜观察和RT-PCR鉴定表达S100A13的细胞克隆并检测转染前后细胞FGF1表达量的变化;MTT观察阴性对照组、转染空载体组和转染重组基因组的细胞生长曲线.各组分别加入化疗药物(5-Fu),24h后收集细胞,流式细胞术检测细胞凋亡.结果 转染后的重组细胞系S100A13表达明显增高,转染PcDNA3.1/NT-GFP-S100A13组FGF1的表达量明显高于阴性对照组及空载体组.但各组细胞的生长曲线以及对化疗药物(5-Fu)的敏感性差异无显著性.结论 S100A13及其介导释放的FGF1在肿瘤的增殖及对抗癌药物的敏感性方面均没有明显的作用.
|
| 关 键 词： | S100A13基因 真核表达载体 基因转染 |
Overexpression of S100A13 in HEPG2 cells and its biological characteristics |
| |
| LIU Ying-lan , YANG Jing , ZHONG Jing , CAO Ren-xian. Overexpression of S100A13 in HEPG2 cells and its biological characteristics[J]. China Journal of Modern Medicine, 2011, 21(16) |
| |
| Authors: | LIU Ying-lan YANG Jing ZHONG Jing CAO Ren-xian |
| |
| Affiliation: | LIU Ying-lan,YANG Jing,ZHONG Jing,CAO Ren-xian(Department of Endocrinology,the First Affiliated Hospital of Nanhua University,Hengyang,Hunan 421001,P.R.China) |
| |
| Abstract: | [Objective] To research the biological characterization of HEPG2 cell line transfected with S100A13 gene.[Methods] The recombinant PcDNA3.1/NT-GFP-S100A13 was transfected into HEPG2 cells by lipofectamine,the mRNA expression level of S100A13 and FGF1 were identified by RT-PCR,respectively.The cell proliferation status was examined by MTT technical.Then cells were treated with 5-Fu.The cell apoptosis rates were detected by flow cytometry.[Results] S100A13 was stably expressed in transfected cell line and the... |
| |
| Keywords: | S100A13 gene eukaryotic expression vector gene transfection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
