Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR |
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Authors: | Nagao Shoji Yoshinouchi Mitsuo Miyagi Yasunari Hongo Atsushi Kodama Junichi Itoh Sachio Kudo Takafumi |
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Institution: | Department of Obstetrics and Gynecology, Okayama University Medical School, Okayama, Japan. |
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Abstract: | A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA. |
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