| 慢性低氧高二氧化碳大鼠支气管骨桥蛋白及其基因的表达 |
| |
| 引用本文： | 施孟如,林全,王良兴,陈少贤.慢性低氧高二氧化碳大鼠支气管骨桥蛋白及其基因的表达[J].中国老年学杂志,2006,26(12):1695-1696. |
| |
| 作者姓名： | 施孟如 林全 王良兴 陈少贤 |
| |
| 作者单位： | 1. 温州医学院生物学实验教学中心,浙江,温州,325003
2. 温州医学院附属第一医院呼吸内科 |
| |
| 摘 要： | 目的研究骨桥蛋白（OPN）基因和蛋白在慢性低氧高二氧化碳大鼠的支气管表达情况。方法采用免疫组化和原位杂交技术检测大鼠支气管OPN蛋白和mRNA表达情况。结果OPN蛋白和mRNA在低氧高二氧化碳组大鼠支气管上皮细胞及平滑肌细胞中广泛表达，与对照组比较均有显著性差异（P〈0．01）。结论慢性低氧高二氧化碳诱导了大鼠支气管骨桥蛋白的表达。
|
| 关 键 词： | 支气管 骨桥蛋白 低氧 慢性 高碳酸血症 |
| 文章编号： | 1005-9202（2006）12-1695-02 |
| 收稿时间： | 2006-02-17 |
| 修稿时间： | 2006-04-10 |
Expression of osteopontin protein and its gene in the bronchi of chronic hypoxic hypercapnic rats |
| |
| SHI Meng-Ru, LIN Qmm, WANG Liang-Xing,et al..Expression of osteopontin protein and its gene in the bronchi of chronic hypoxic hypercapnic rats[J].Chinese Journal of Gerontology,2006,26(12):1695-1696. |
| |
| Authors: | SHI Meng-Ru LIN Qmm WANG Liang-Xing |
| |
| Institution: | Central Laboratory of Biology, Wenzhou Medical College, Wenzhou 325003, Zhejiang, China |
| |
| Abstract: | Objective To study the role of the gene and protein of expression of osteopontin(OPN) in the bronchi of chronic hypoxic hypercapnic rats.Methods OPN gene and protein expression in bronchi were detected by in situ hybridization and immunohistochemistry respectively.Results OPN gene and protein widely expressed in epithelia and smooth muscle cells of bronchi in rats of hypoxic hypercapnia group having significant difference with those of control(P<0.01).Conclusions Expression of OPN gene and protein in bronchi of rats may be induced by chronic hypoxic hypercapnia. |
| |
| Keywords: | Bronchi Osteopontin(OPN) Hypoxic chronic Hypercapnia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
