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三种方法检测孕妇血清IgG抗A(B)效价的结果比较
引用本文:张庆侠,郝建华,倪琳婷,程琼,周天祥.三种方法检测孕妇血清IgG抗A(B)效价的结果比较[J].实用医技杂志,2008,15(5).
作者姓名:张庆侠  郝建华  倪琳婷  程琼  周天祥
作者单位:深圳市第六人民医院,广东深圳,518052
摘    要:目的:比较经典法、改良法和微柱凝胶法抗人球蛋白试验检测孕妇血清中IgG抗A(B)效价的差异。方法:用经典法、改良法和微柱凝胶法分别检测136例孕妇血清中IgG抗A(B)效价,计算其阳性率,再以经典法为标准计算改良法和微柱凝胶法的特异性和敏感性。结果:经典法、改良法和微柱凝胶法检测孕妇血清中IgG抗A效价的阳性率分别为22.8%、25.0%和33.8%,IgG抗B效价的阳性率分别为19.1%、20.5%和31.6%。与经典法比较,改良法敏感性为98.1%,特异性为96.3%;微柱凝胶法敏感性为100%,特异性为63.4%。经统计学比较,,改良法和经典法的阳性率无统计学意义(P>0.05),而微柱凝胶法阳性率明显高于经典法(P<0.01)。结论:改良法和经典法检测孕妇血清中IgG抗A(B)效价的结果具有良好的一致性,而微柱凝胶法阳性率明显高于经典法,需重新设定临床意义决定值。

关 键 词:孕妇  IgG抗A(B)效价  抗人球蛋白试验  经典  改良  微柱凝胶

Comparing the Results of the Determination of Anti-A (B) IgG Titers with Three Assays in the Pregnant Women
ZHANG Qing-xia,HAO Jian-hua,NI Lin-ting,CHENG Qiong,ZHOU Tian-xiang.Comparing the Results of the Determination of Anti-A (B) IgG Titers with Three Assays in the Pregnant Women[J].Journal of Practical Medical Techniques,2008,15(5).
Authors:ZHANG Qing-xia  HAO Jian-hua  NI Lin-ting  CHENG Qiong  ZHOU Tian-xiang
Institution:ZHANG Qing-xia; HAO Jian-hua; NI Lin-ting; CHENG Qiong; ZHOU Tian-xiang(The Sixth People s Hospital of Shenzhen; Shenzhen; Guangdong 518052; China);
Abstract:Objective To study comparatively the different of micro-column gel(MCG),improvement and classic Coombs,test in the determination of anti-A(B)IgG titers of the pregnant women.Methods Sera were obtained from 136 pregnant women,Anti-A(B)IgG titers were determined respectively by the MCG,improvement and classic Coombs,test.The positive rate,sensitivity and specificity of three assays were evaluated.Results The positive rate of anti-A IgG titers with MCG,improvement and classic Coombs,test were separately 33.8%、...
Keywords:Pregnant women  Anti-A(B)IgG titers  Coombs test  Classic  Improvement  Micro-column gel
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