GFAP和Fos蛋白在戊四氮致痫大鼠前脑中的表达变化

目的 研究大鼠在戊四氮导致癫痫发作时前脑内星形胶质细胞和神经元的形态学反应及其相互关系。方法 应用免疫组织化学单标记法分别显示前脑内GFAP和Fos蛋白表达的时间规律，并用免疫组织化学双重标记显示GFAP和Fos蛋白表达的相互关系。结果 在戊四氮导致大鼠癫痫发作早期，前脑的星形胶质细胞被激活，细胞体积增大，突起粗大，GFAP表达阳性，随着存活时间的变化，星形胶质细胞的反应经历先逐渐升高后降低的过程。被激活的星形胶质细胞和神经元表达Fos蛋白阳性，也呈现逐渐升高又降低的变化；另外，GFAP阳性星形胶质细胞和Fos阳性神经元在前脑主要分布在大脑皮层、海马、杏仁核等部位，二者的分布特征基本一致。结论 星形胶质细胞可能和神经元一起参与了戊四氮所致癫痫发作的变化。

Change in expression of GFAP and Fos protein in forebrain of pentylenetetrazol (PTZ)-induced seizure in rats

Objective To observe the early morphological response of astrocytes and neurons and their relationship with each other in forebrain of pentylenetetrazol (PTZ)-induced seizure in rats. Methods By using the single-labeled immunohistochemical staining method, we investigated the time course of expression of GFAP or Fos in forebrain. The double-labeled immunohistochemical staining for anti-GFAP and anti-Fos was used to investigate the relation of GFAP positive cells and Fos positive cells. Results In the early stage(from 15 to 360 min)of seizure, the astrocytes were activated, its bodies hypertrophied and processes thickened, and expressed GFAP positive. GFAP-like immunoreactivity(-LI) was increased first, then decreased and disappeared finally;Activated Fos-LI neurons and astrocytes were found in the forebrain, and first increased, then decreased and disappeared finally too. GFAP-LI astrocytes and Fos-LI neurons mostly distributed in cerebral cortex, hippocampus and amygdaloid nucleus. Conclusion The present results indicate that the astrocyte together with the neuron may be involved in the early change of seizure.