Skeletal muscle ATP turnover and single fibre ATP and PCr content during intense exercise at different muscle temperatures in humans |
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Authors: | Stuart R Gray Karin Soderlund Moira Watson Richard A Ferguson |
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Institution: | (1) Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, UK;(2) Swedish School of Sport and Health Sciences, Stockholm, Sweden;(3) Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK;(4) School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK |
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Abstract: | The effect of temperature on skeletal muscle ATP turnover, pulmonary oxygen uptake and single fibre ATP and PCr content was
studied during intense cycling exercise. Six healthy male subjects performed 6-min intense (Δ50%LT-VO2peak) cycling, at 60 rpm, under conditions of normal (N) and elevated muscle temperature (ET). Muscle biopsies obtained from the
vastus lateralis at rest, 2 and 6 min were analysed for homogenate ATP, PCr, lactate and glycogen, allowing estimation of
anaerobic ATP turnover. Freeze-dried single fibres from biopsies were characterised according to their myosin heavy chain
composition (type I, IIA or IIAX) and analysed for ATP and PCr content. Pulmonary gas exchange was measured throughout. There
was no difference in pulmonary oxygen uptake between the trials. The elevation of muscle temperature resulted in a lower (P < 0.05) PCr content, higher (P < 0.05) lactate content and greater (P < 0.05) anaerobic ATP turnover after 2 min of exercise. There was no effect of temperature on these measures at 6 min. In
single fibres it was observed that in ET, there was a lower (P < 0.05) PCr content in type I fibres after 2 min with no differences between conditions after 6 min. The present study demonstrates
that elevation of muscle temperature results in a greater anaerobic ATP turnover and type I fibre PCr degradation during the
initial 2 min of intense exercise. |
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