经肝动脉化疗栓塞术对肝癌细胞生存素和增殖细胞核抗原的影响

目的 研究经肝动脉化疗检塞术(TACE)对肝癌细胞生存素mRNA、蛋白和增殖细胞核抗原的影响.方法 设术前行TACE组(A组)、术前未行TACE组(B组)和正常肝组织组(C组).检测肝组织中生存素mRNA、蛋白和增殖细胞核抗原的表达.结果 ①A组癌组织生存素mRNA阳性表达率和灰度差值均低于B组,差异有统计学意义(均P<0.05).②A组癌组织生存素蛋白阳性表达率和灰度差值均低于B组,差异有统计学意义(均P<0.05).③B组癌组织的增殖指数高于A组和C组(均P<0.05).结论 肝癌组织中生存素mRNA及其蛋白有较高的阳性表达率,TACE能降低癌组织生存素mRNA及其蛋白的表达,抑制增殖活性.

Effect of preoperative transcatheter arterial chemoembolization on Inhibitor of apoptosis protein-Survivin and Survivin mRNA of hepatocellular carcinoma.

Objective To study the effect of transcatheter arterial chemoembolizafion (TACE) on inhibitor apoptosis protein-Survivin, Survivin mRNA and proliferating cell nuclear antigen (PCNA). Methods Group A of hepatocelluar carcinoma with preoperative TACE (n = 26), group B of hepatocelluar carcinoma without any interven-tional therapy (n = 30) ,group C of Normal liver (n = 16) were divided. The expression of Survivin mRNA and the expression of Survivin and PCNA were detected. Result ①The positive Survivin mRNA expression in group A and the grey difference were lowere than that in group B (P < 0.05) ; ②The positive Survivin expression and the grey difference in group A were lower than that in group B (P < 0.05) ; ③The proliferating index (PI) of carcinoma tis-sure in group B was higher in group A and group C (P < 0.05 for each). Conclusion There is a high positive ex-pression of Survivin mRNA and Survivin, which may be decreased by TACE and then the proliferation of hepatocellu-lar carcinoma cell is surpressed.