黏着斑激酶的高表达与人胃癌侵袭和转移的关系

目的 探讨胃癌侵袭和转移过程中黏着斑激酶（FAK）的表达与其生物学行为的关系。方法 用免疫组化SABC法检测10例行胃癌根治术所收集的癌组织、癌旁组织、正常胃黏膜组织和胃区域淋巴结标本中FAK的表达情况。结果FAK表达强阳性率在胃癌组织中（78．0％）明显高于正常胃黏膜组织（10．0％）及癌旁组织（20．0％），P〈0．01，正常胃黏膜组织与癌旁组织中的表达差异无统计学意义（P〉0．05）。FAK表达强阳性率在淋巴结有转移和无转移患者中分别是68．8％（22／32）和33．3％（6／18），前者明显高于后者（P〈0．05）；在有转移的淋巴结中和无转移的淋巴结中分别是87．2％（136／156）和40．9％（18／44），前者明显高于后者（P〈0．01）；FAK表达强阳性率与胃癌浸润深度和分化程度有关，浸润深度越深，细胞分化越低，表达强阳性率越高（P〈0．05）；与Borrmann分型、肿瘤部位及大小无关（P〉0．05）。结论 FAK表达与胃癌发生、发展有关，FAK表达增高是胃癌细胞浸润和转移的重要原因之一，检测癌组织中FAK表达水平，有助于进一步了解胃癌的生物学行为，有利于治疗与预后判断。

Relationship Between Strong Immunoreactivity of Focal Adhesion Kinase and Invasion and Metastasis in Human Gastric Carcinoma

Objective To study the correlation between focal adhesion kinase (FAK) expression and biological behavior of invasion and metastasis in gastric carcinoma. Methods The immunoreactivity of FAK was revealed by immunohistochemical method in gastric carcinoma tissues,cancer-adjacent tissues, normal gastric mucosa,and 200 regional lymph nodes in 50 collected specimens when radical resection of gastric carcinoma were carried out. Results The percentage of FAK strongly positive immunoreactivity were 10.0%(5/50), 20.0%(10/50) and 78.0%(39/50) in normal gastric mucosa, cancer-adjacent tissues and gastric carcinoma tissues respectively. The strong immunoreactivity was obviously higher in gastric carcinoma tissues than that in normal gastric mucosa or cancer-adjacent tissues (P<0.01). There were no difference between normal gastric mucosa and cancer-adjacent tissues (P> 0.05). The percentage of strongly positive immunoreactivity of FAK were 68.8%(22/32) and 33.3%(6/18) in cases with metastasis and without metastasis of lymph node respectively. The immunoreactivity of FAK in cases with metastasis of lymph node was significantly higher than that without metastasis of lymph node (P<0.05).The strong immunoreactivity of FAK were 87.2%(136/156) and 40.9%(18/44) in with-metastatic lymph node and without-metastatic lymph node. The immunoreactivity of FAK in with-metastatic lymph node was significantly higher than that without-metastatic lymph node (P<0.01). The percentage of FAK strongly positive immunoreactivity showed correlation with the cellular differentiation and depth of infiltration of gastric carcinoma. The deeper infiltration and lower differentiation, the stronger expression rate was obtained (P<0.05), which showed no correlation with Borrmann type, location and size of tumor (P>0.05). Conclusion Increased immunoreactivity of FAK is an important role of invasion and metastasis for gastric carcinoma cells. Detection of FAK expression in cancer tissues can be helpful to understand the carcinogenic biological behavior of gastric carcinoma as well as to make judgment and treatment of prognosis of patients.