Stress-induced reduction in the rat mixed lymphocyte reaction is due to macrophages and not to changes in T cell phenotypes |
| |
Authors: | M. Fleshner D. Bellgrau L.R. Watkins M.L. Laudenslager S.F. Maier |
| |
Affiliation: | aDepartment of Psychology, University of Colorado-Boulder, Campus Box 345, Boulder, CO 80309, USA;bDepartment of Microbiology / Immunology, University of Colorado Health Sciences Center, Boulder, CO, USA;cDepartment of Psychiatry, University of Colorado Health Sciences Center, Boulder, CO, USA |
| |
Abstract: | Exposure to aversive events or Stressors modulates various aspects of immune function. We have previously reported that exposure to an acute Stressor, inescapable tail shock (IS), resulted in a shift in T cell subpopulations in rat mesenteric lymph nodes but not in cervical lymph nodes (Fleshner et al. (1992) J. Neuroimmunol. 41, 131–142). The mesenteric ratio was increased immediately after exposure to IS and was due primarily to an increase in the percent of CD4+ cells. The present experiments were designed to determine the relationship between the IS-associated phenotypic shift and its significance in the function of CD4+ T cells. The function assessed was the in vitro proliferative response to alloantigens coded for by the Major Histocompatibility Complex (MHC). Using the mixed lymphocyte reaction (MLR), we report that exposure to IS resulted in a decrease in the MLR response of cells from both cervical and mesenteric lymph nodes. Depletion of macrophages (nylon wool adherent cells) eliminated the IS-induced reduction and co-culture of macrophages (irradiation-insensitive cells) from shocked rats produced the suppression. One interpretation of these data is that exposure to IS resulted in the activation of macrophages and the release of a suppressive factor which reduced the MLR response of peripheral lymph node lymphocytes. |
| |
Keywords: | Stress Mixed lymphocyte reaction Lymphocyte subpopulations CD4+ T cells |
本文献已被 ScienceDirect 等数据库收录! |
|