再细胞化牛心包纤维支架试片体内移植:1年的动物实验研究(英文)

目的进一步观察移植后内皮细胞（EC）抗切应力和抗钙化能力以及肌成纤维细胞迁移和自身修复能力。方法新鲜牛心包片经脱细胞、鞣制、改性后备用；A、B组试片上依序种植宿主肌成纤维细胞和EC，C组试片上不种植细胞，随后将3组试片分别移植于猪的腹主动脉壁上；2个月后对A组试片，12个月后对B、C组试片进行厚度、钙含量、扫描电镜及组织学的检测。结果A、B组试片钙含量显著低于C组（P〈0．05），但显著高于新鲜牛心包（P〈0．05）；A、B组试片钙含量差异无显著性（P〉0．05）；A、B组试片显著厚于C组试片（P〈0.05），A、B组试片的厚度差异无显著性（P〉0．05）；B组试片80％～90％的表面覆盖EC，肌成纤维细胞已迁移至表层下支架的3／4；C组试片表面无细胞，肌成纤维细胞已迁移至表层下支架的1／4～1／3，大部分胶原纤维间隙消失。结论人工EC化使生物材料抗钙化能力增强；种植肌成纤维细胞使生物材料具有自身修复能力。但增厚的瓣叶是否影响其功能，尚有待进一步研究。

Implantation of recellularized pericardial fibrous scaffold in vivo:a one-year animal study

Objective To further analyze the ability of resisting the shear-stress and anti-calcification of endothelial cells (EC),and further analyze the ability of migrating and self-repair of myofibroblasts. Methods The fresh bovine pericardia were acellularized,tanned and modified; Autologous myofibroblasts and ECs were seeded on the patches of Group A and B sequentially; Group C was unseeded control group. Then the patches of all groups were implanted into the porcine abdominal aortic wall separately; The retriev...