鬼针草总黄酮对实验性脑出血大鼠脑损伤的保护作用

摘要] 目的：观察鬼针草总黄酮（TFB）对大鼠实验性脑出血后脑组织损伤的影响，探讨其对大鼠脑损伤的保护机制。方法：采用大脑立体定位技术，取不抗凝血液50ul缓慢注入尾状核建立大鼠实验性脑出血模型，观察TFB对脑组织含水量、全血粘度及脑匀浆中超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）等生化指标的影响。结果：TFB高、中、低剂量组均能显著改善脑出血大鼠脑含水量、全血粘度，高、中剂量组能明显升高脑组织SOD活性，降低MDA、NO含量(P〈0.05)。结论：TFB对实验性脑出血大鼠脑损伤的保护作用可能与其减轻脑水肿、改善血肿周围组织的微循环状态、减轻细胞膜脂质过氧化损伤、抑制NO的产生有关。

Neuroprotection of Total Flavones of Bidens Bipinnata L. on Brain Damage Following Experimental Intracerebral Hemorrhage in Rats

Objective To investigate the neuroprotective and mechanism of total flavones of bidens bipinnata L.（TFB） on brain damage following experimental intracerebral hemorrhage in rats.Methods Experimental intracerebral hemorrhage was induced in rats through infusion 50 L of fresh autologous blood drawn into the caudate uncleus.The brain water content,whole blood viscosity and the level of SOD,MDA and NO were measured.Results TFB（100mg·kg-1、30mg·kg-1、10mg·kg-1） significant improve the brain water content and status whole blood viscosity.The level of MDA,NO was lower,and the level of SOD was obviously higher in high-,middle-dose TFB group in cerebral tissue（P 0.05）.Conclusion TFB has evident protective effect on brain damage after experimental intracerebral hemorrhage in rats,which maybe related to diminished cerebral edema,improve the microcirculation status of perihematomal penumbra,the increased the ability of decreased lipid peroxidation,inhibited production of NO.