Abnormal chromosome behavior in human oocytes which remained unfertilized during human in vitro fertilization |
| |
Authors: | Horst Spielmann Christiane Krüger Manfred Stauber Richard Vogel |
| |
Institution: | (1) Max von Pettenkofer Institut des Bundesgesundheitsamtes, P.O. Box 33 oo 13, D-1000 Berlin 33, West Germany;(2) Department of Gynaecology and Obstetrics of the Freie Universität Pulsstr, D-1000 Berlin 19, West Germany |
| |
Abstract: | Chromosomal abnormalities and abnormal embryonic development have previously been observed after human in vitro fertilization (IVF). Chromosomal abnormalities may arise not only after fertilization but even earlier during meiotic maturation of human oocytes in culture. Since chromosomal analysis is simple in oocytes during meiotic maturation, the chromosomal status was analyzed in oocytes which remained unfertilized in a human in vitro fertilization program. In 50 fertilization attempts the chromosomes of 62 unfertilized oocytes could be analyzed; 45 of them were in the process of meiotic maturation. In three oocytes two small polar bodies were observed 16–18 hr after insemination in the absence of fertilization. In one oocyte abnormal chromosome behavior was found during the first meiotic division, and in four oocytes during metaphase of the second meiotic division. These data suggest that chromosomal analysis of unfertilized oocytes in human IVF may improve the understanding of human oocyte maturation and fertilization. |
| |
Keywords: | human IVF abnormal oogenesis cytogenetical analysis |
本文献已被 SpringerLink 等数据库收录! |