黄连同工酶及相关逆境指标对UV-B的响应研究

目的:探讨UV-B辐射胁迫下黄连的抗逆生理机制,为黄连栽培及提高黄连品质提供参考依据。方法:通过设置UV-B辐射时间及不同强度梯度,运用聚丙烯酰胺垂直板电泳技术,对各处理组的黄连同工酶及相关逆境指标进行了分析。结果:UV-B辐射3 h后,SOD1(Rf=0.125)、SOD2(Rf=0.312)、CAT1(Rf=0.428)、POD3(Rf=0.290)、POD4(Rf=0.636)谱带被先后诱导,随着UV-B辐射时间的增长,这些谱带开始丢失或减弱,尤其处于重度UV-B胁迫下,CAT1(Rf=0.428)、POD3(Rf=0.290)谱带会提前丢失;此外,UV-B辐射使得叶片中MDA、可溶性糖、脯氨酸含量显著高于对照组,但UV-B辐射7 h后,除UL组脯氨酸上升外,各组MDA、可溶性糖、脯氨酸含量开始有所下降,可溶性蛋白谱带数增多。结论:短时间的UV-B辐射会诱导黄连增加抗氧化酶同工酶的表达量及谱带数,积累可溶性糖、可溶性蛋白等抗性物质来提高自身抗性能力,UV-B辐射持续7 h后,黄连自身调节系统被破坏,代谢开始紊乱,同工酶谱带开始丢失及减弱,可溶性蛋白条带增多变宽。

Response of isozyme and stress indexes of Coptis chinensis to UV-B radiation

Objective:To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality.Methods:Different degrees of the range of time and intensity of UV-B radiation were set in the experiment.Used the technique of polyacrylamide gelatin vertical board electrophoresis(PAGE) to analyse the isozyme and related stress index.Results:The isoenzymic bands of SOD1(Rf=0.125),SOD2(Rf=0.312),CAT1(Rf=0.428),POD3(Rf=0.290),POD4(Rf=0.636)were induced by UV-B radiation after 3 hours,with the increase of the time of UV-B radiation,those isoenzymic bands was going to vanish or became unclear.Moreover,isoenzymic bands of CAT1(Rf=0.428)、POD3(Rf=0.290) disappeared in advance under heavy intensity of UV-B radiation.Furthermore,the contents of MDA,soluble sugar,proline were higher dramatically than those of control group under UV-B radiation.However,excluding the increases of proline in UL group,the content of MDA,soluble sugar,proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation.Conclusion:The increase of the expression of antioxidase isozyme,accumulation of soluble sugar,soluble protein and other antioxidase matter is induced by the short-time UV-B radiation,which can protect Coptis chinensis from being harmed by UV-B radiation.However,regulation system of Coptis chinensis are broken,metabolism is disordered,the bands of antioxidase isozyme vanish or weaken,the bands of soluble protein are increased and widened,these phenomenon is caused by 7 hours of UV-B radiation.