杜仲饮片HPCE指纹图谱的研究

目的:建立杜仲饮片HPCE指纹图谱分析方法,并对杜仲及其炮制品的指纹图谱进行比较。方法:采用高效毛细管电泳法进行色谱分离,以60 mmol/L硼砂-20 mmol/L磷酸二氢钠-10%甲醇(pH=10.0)为运行缓冲液,分离电压为20 kV,波长为210 nm,以松脂醇二葡萄糖苷为参照物,测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以10个共有峰为特征指纹信息的杜仲饮片HPCE指纹图谱;发现少数杜仲饮片的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:该方法准确可靠,重现性好,可作为杜仲饮片内在质量评价的依据。

Study on HPCE fingerprint of Eucommiae Cortex

Objective: To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE and compare the fingerprints of Eucommiae Cortex and its processed products.Methods: Based on the mode of high performance capillary electrophoresis,60 mmol/L sodium borate-20 mmol/L sodium dihydrogen phosphate-10% methanol(pH 10.0) was used as buffer solution.The separation voltage was 20 kV and the detection wavelength was set at 210 nm.Pinoresinol diglucoside was used as a reference standard,the chromatographic fingerprint were determined.The data were analyzed by fuzzy cluster and fingerprint similarity evaluation softwarewas used to compare the similarity of samples.Results: HPCE fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily.It was discovered that a small number of samples differed from others.Regarding to the fingerprints of Eucommiae Cortex and its processed products,there were obvious differences in the relative areas of common peaks.Conclusion: The method is reliable,accurate and can be used for quality control of Eucommiae Cortex.