莪术挥发油的提取工艺优化及质量分析

目的:考察提取-共沸精馏耦合工艺(WER)与水蒸气蒸馏工艺(SD)及提取条件对莪术挥发油得率的影响,确定最佳工艺和条件。方法:WER与SD两种工艺分别采用正交试验方法,并采用配对T检验法评价莪术挥发油得率差异的显著性,对莪术挥发油进行GC-MS分析和比较。结果:WER与SD工艺的最佳提取条件为:超声0h,提取12 h,8倍量水(SD:12倍量水),两种工艺下莪术挥发油得率有显著性差异,其挥发油中有12个共有组分,分别占鉴定出组分的97.19%(SD)与92.25%(WER),且共有组分的含量差异不大。结论:超声不利于莪术挥发油提取,相同条件下WER提取的莪术挥发油可延续SD工艺下挥发油的品质且能显著提高莪术挥发油得率。

Extraction technology optimization and quality analysis of volatile oil in rhizoma curcumae

Objective:To investigate the effect of technologies and conditions on volatile oil yield extracted from Rhizoma Curcumae.Methods: Water Extraction coupling Rectification(WER) and Steam Distillation(SD) technologies were applied to extract the volatile oils based on orthogonal table L9(33) to find out optimized condition.Results: Variance and range analysis of orthogonal experiment results showed that the best conditions of WER and SD were as follows: ultrasound 0 h,extract 12 h with 8(or 12 fold water for SD) fold water amount.Paired T test on the yields of the oils indicated that the oil yields prepared by WER and SD were significantly different.GC-MS analysis characterized 12 common compounds,which occupied 97.19%(SD) and 92.25%(WER) of the ones identified,respectively.Moreover,the relative percentage of the common constituents were almost the same.Conclusion: Ultrasound is not good for extracting volatile oil from Rhizoma Curcumae.WER could not only increase the oil yield of Rhizoma Curcumae,but also keep the quality of the oils accord with that extracted by SD.