EGCG对顺铂损伤HEK293细胞及杀伤A549细胞的影响

目的: 探讨表儿茶素没食子酸酯(EGCG)对顺铂(DDP)致人胚肾HEK293细胞损伤及对DDP杀伤人肺癌A549细胞的影响。方法: 体外培养HEK293细胞和A549细胞,分为对照组、DDP组和DDP+EGCG组,DDP组和DDP+EGCG组同时建立DDP损伤模型,MTT法检测EGCG和/或DDP对HEK293细胞和A549细胞存活率的影响。结果: EGCG对HEK293细胞的IC₅₀值为61.6 mg/L。当EGCG浓度<40 mg/L时,对DDP诱导的HEK293细胞损伤没有显著性影响,EGCG浓度≥40 mg/L时,可显著性增强DDP对HEK293细胞的损伤作用。EGCG对A549细胞的IC₅₀值为33.6 mg/L。当EGCG浓度≥32 mg/L时,可显著增强DDP对A549细胞的杀伤作用。结论: EGCG对DDP所致HEK293细胞损伤无保护作用,但EGCG对癌细胞毒性作用大于其对正常细胞的毒性,且当EGCG与DDP同时使用时可以加重A549细胞损伤。

Effects of epigallocatechin gallate on cisplatin-induced cytotoxicity in HEK293 and A549 cell lines

OBJECTIVE: To investigate the effects of epigallocatechin gallate (EGCG) on cisplatin (DDP)-induced cytotoxicity in human embryo kidney (HEK) 293 cells and A549 cells. METHODS: HEK293 cells and A549 cells were cultured in vitro,and effects of DDP and EGCG on HEK293 cells and A549 cells were observed by MTT. The cells were divided into control group,DDP group and DDP+EGCG group. Then,the rates of HEK293 and A549 cell death were investigated by MTT. RESULTS: The IC₅₀ of EGCG on HEK293 cells was 61.6 mg/L. At lower than 40 mg/L,EGCG had no significantly effects on DDP-induced HEK239 cell death. At higher than 40 mg/L,EGCG significantly enhanced DDP-induced HEK239 cell death. The IC₅₀ of EGCG on A549 cells was 33.6 mg/L. At higher than 40 mg/L,EGCG significantly enhanced DDP-induced A549 cell death. CONCLUSION: EGCG had no significant protective effects on DDP-induced HEK239 cell death,but EGCG induced more cytotoxicity on cancer cells than on normal cells. When co-treated with DDP,A549 cell sustained more damage than HEK239 cells.