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PHI-like immunoreactivity co-locates with the VIP-containing system in human lumbosacral spinal cord
Authors:P Anand  S J Gibson  Y Yiangou  N D Christofides  J M Polak  S R Bloom
Affiliation:Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, D-7400 Tübingen F.R.G.
Abstract:We report here that, in comparison to aggregates from retinal cells alone, addition of pigmented epithelial cells to retinal cells in rotary culture results in a pronounced increase of spatial order. A particularly high level of organization is found in about 15-20% of the aggregates. In these 'retinoids' the main layers characteristic of developing in vivo retinae can be distinguished in correct sequential arrangement on the basis of morphological criteria and by using acetylcholinesterase histochemistry [5, 6, 15], peanut agglutinin-lectin binding [11] and Lucifer Yellow staining [7-9].
Keywords:chick retina  cell aggregates  sorting out  lamination  retinoids  pigment epithelium
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