| HBeAg特异性细胞免疫反应体外抗乙肝病毒作用 |
| |
| 引用本文： | 陈瑾,吴金明,林贤凡,孙慧,金思思,申苏建. HBeAg特异性细胞免疫反应体外抗乙肝病毒作用[J]. 胃肠病学和肝病学杂志, 2010, 19(4) |
| |
| 作者姓名： | 陈瑾 吴金明 林贤凡 孙慧 金思思 申苏建 |
| |
| 作者单位： | 1. 台州市立医院儿科,浙江,台州,318000
2. 温州医学院附属第一医院消化内科 |
| |
| 基金项目： | 温州市2009年科技计划项目 |
| |
| 摘 要： | 目的探讨体外HBeAg特异性细胞免疫反应对乙型肝炎病毒(HBV)mRNA表达及蛋白合成的影响。方法自正常人外周血单核细胞中诱导培养树突状细胞(DC),经不同浓度的HBeAg负载后与自身T细胞共同培养,诱导产生HBeAg特异性T细胞,同时设与无HBeAg刺激的DC共同培养的T细胞、单纯T细胞为对照组,作用于HepG2.2.15细胞系,逆转录PCR(RT-PCR)检测HepG2.2.15细胞内乙肝表面抗原(HBsAg)、乙肝核心抗原(HBcAg)的mRNA水平,酶联免疫法(ELISA)检测细胞培养上清中HB-sAg和乙肝e抗原(HBeAg)水平。结果经HBeAg刺激激活的T细胞可以有效地抑制HBsAg、HBcAg mRNA的表达(P0.05)及HBsAg、HBeAg的合成(P0.05),且随着HBeAg刺激浓度的升高,对上述指标的抑制率逐渐上升。结论HBeAg特异性细胞免疫反应可显著抑制HBV mRNA和蛋白的表达,为开发HBeAg相关的治疗性疫苗奠定理论基础。
|
| 关 键 词： | 乙型肝炎病毒 树突状细胞 细胞免疫 HepG2.2.15细胞 |
Anti-HBV effect of HBeAg specific cellular immune response in vitro |
| |
| CHEN Jin,WU Jinming,LIN Xianfan,SUN Hui,JIN Sisi,SHEN Sujian. Anti-HBV effect of HBeAg specific cellular immune response in vitro[J]. Chinese Journal of Gastroenterology and Hepatology, 2010, 19(4) |
| |
| Authors: | CHEN Jin WU Jinming LIN Xianfan SUN Hui JIN Sisi SHEN Sujian |
| |
| Affiliation: | CHEN Jin1,WU Jinming2,LIN Xianfan2,SUN Hui2,JIN Sisi2,SHEN Sujian2 1.Department of Pediatrics,Taizhou Municiple Hospital,Taizhou 318000,2.Department of Gastroenterology,the First Affiliated Hospital of Wenzhou Medical College,China |
| |
| Abstract: | Objective To investigate the effect of HBeAg specific cellular immune response on hepatitis B virus mRNA and protein expression in vitro.Methods The monocyte-derived dendritic cells(DC) were generated from healthy people and cocultured with self-blood T lymphocyte after loaded with different concentration of HBeAg to induce specific T lymphocyte.While DC without HBeAg loaded group,only T lymphocyte group were established as control group.HepG2.2.15 cells and culture supernatant were collected after stimulat... |
| |
| Keywords: | Hepatitis B virus Dendritic cells Cellular immune HepG2.2.15 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
