| HPLC法测定维生素B1的含量 |
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| 引用本文： | 丁新爱,王新春. HPLC法测定维生素B1的含量[J]. 河南大学学报(医学版), 2005, 24(3): 27-28 |
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| 作者姓名： | 丁新爱 王新春 |
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| 作者单位： | 河南省医药技师学院,河南,开封,475003;河南大学东京医院,药械科,河南,开封,475001 |
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| 摘 要： | 目的:建立HPLC法测定维生素B1的含量。方法:选用岛津C18(150mm×4.6mm,5μm),乙腈-水(辛烷基磺酸钠0.32g、三乙胺2mL、冰醋酸2mL加水至1000mL)(13:87)为流动相;检测波长为254nm;流速:1.0mL/min。结果:维生素B1在5.2μg/mL、~26μg/mL范围内,线性关系良好,回归方程:Y=16855×X-1979,r=0.9998。平均回收率101.75%(n=9)。结论:本法准确、灵敏、简便。
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| 关 键 词： | 反相高效液相色谱法 维生素B1 含量 |
| 文章编号： | 1672-7606(2005)03-0027-02 |
| 收稿时间： | 2005-03-10 |
| 修稿时间： | 2005-03-10 |
Determination of the vitamin B1 contents with contra- phase HPLC |
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| DING Xin-ai,WANG Xin-chun. Determination of the vitamin B1 contents with contra- phase HPLC[J]. Journal of Henan University, 2005, 24(3): 27-28 |
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| Authors: | DING Xin-ai WANG Xin-chun |
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| Affiliation: | DING Xin-ai~1,WANG Xin-chun~2 |
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| Abstract: | Objective: To establish the method of determining the vitamin B1 contents with HPLC.Methods: DAO JING C was 18(150mm×4.6mm,5μm), the mobile was acetonitrile-water(octane sulfonic natrium0.32 g, tri-ethylamine2ml,glacial acetic acid 2ml added in 1000ml) (13:87),the flow rate was 1.0 ml/min,the detection wavelength was at 254nm. Results: The linear range of vitamin B1 was in the range of 5.2μg/mL、~26μg/mL,linear relation was good, the regression equation was Y=16855×X-1979,r=0.9998, and its average recovery was 101.75%(n=9).Conclusion: It is accurate, sensitive and simple. |
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| Keywords: | contra - phase HPLC vitamin B1 contents |
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