| 静态与动态下脱细胞猪主动脉瓣种植人成纤维细胞初步研究 |
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| 引用本文： | 廖中凯,胡盛寿,周建业,阎鹏.静态与动态下脱细胞猪主动脉瓣种植人成纤维细胞初步研究[J].生物医学工程与临床,2002,6(2):61-63. |
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| 作者姓名： | 廖中凯 胡盛寿 周建业 阎鹏 |
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| 作者单位： | 中国医学科学院,中国协和医科大学,阜外心血管病医院瓣膜研究室,北京,100037 |
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| 基金项目： | 国家自然科学基金资助项目 (39970 731 ) |
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| 摘 要： | 目的 探索在脱细胞猪主动脉瓣膜材料上种植人成纤维细胞的方法与效果；材料和方法 采用在脱细胞猪主动脉瓣膜材料上静态与动态种植人成纤维细胞，并对其结果进行比较研究。结果 静态种植不能在猪主动脉瓣叶的两侧形成完整的单层人成纤维细胞融合层；而经过动态种植，在一定条件下可以在猪主动脉瓣叶两侧形成完整的单层人成纤维细胞融合层；结论 动态种植较之静态种植易达到形成完整均匀的细胞种植效果。
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| 关 键 词： | 静态 动态 脱细胞猪主动脉瓣 人成纤维细胞 细胞种植 旋转生物反应器 组织工程瓣膜 |
| 文章编号： | 1009-7090(2002)02-0061-03 |
| 修稿时间： | 2002年3月11日 |
Human fibroblast cell seeding on acellular porcine aorta valves |
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| LIAO Zhong-kai,HU Sheng-shou,ZHOU Jian-ye,YAN Peng Valvular Laboratory of Fuwai Heart Hospital,Chinese Medical Science Academy,Chinese Union Medical College,Beijing ,China.Human fibroblast cell seeding on acellular porcine aorta valves[J].Biomedical Engineering and Clinical Medicine,2002,6(2):61-63. |
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| Authors: | LIAO Zhong-kai HU Sheng-shou ZHOU Jian-ye YAN Peng Valvular Laboratory of Fuwai Heart Hospital Chinese Medical Science Academy Chinese Union Medical College Beijing China |
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| Institution: | LIAO Zhong-kai,HU Sheng-shou,ZHOU Jian-ye,YAN Peng Valvular Laboratory of Fuwai Heart Hospital,Chinese Medical Science Academy,Chinese Union Medical College,Beijing 100037,China |
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| Abstract: | Objective The methods and outcomes of human fibroblast cell seeding on acellular porcine aorta valves were evaluated. Materials and Methods The outcomes of static and dynamic cell seeding was compared. Results Single static seeding can't form a confluent layer of fibroblast cell on either side of aortic valvular leaflet, but static and dynamic seeding can get a confluent layer on either side of it. Conclusion Dynamic cell seeding has advantage over static cell seeding. |
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| Keywords: | acellular porcine aorta valves human fibroblast cell cell seeding rotating bioreactor |
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