| 应用免疫荧光搭桥法检测小鼠肠粘膜内特异性抗体形成细胞 |
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| 引用本文： | 高建新,王焕扭,谢根甫. 应用免疫荧光搭桥法检测小鼠肠粘膜内特异性抗体形成细胞[J]. 中国免疫学杂志, 1987, 0(4) |
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| 作者姓名： | 高建新 王焕扭 谢根甫 |
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| 作者单位： | 上海第二医科大学免疫所，苏州医学院微生物学教研室，苏州医学院微生物学教研室 苏州，苏州 |
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| 摘 要： | 肠粘膜固有膜内特异性抗体形成细胞(ACC)的检测是研究肠道粘膜免疫应答机理的重要指标之一,组织免疫荧光技术是检测粘膜固有膜内特异性ACC的主要方法。目前,在粘膜免疫应答机理的研究中,主要
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A simplified immunofluorescence technique for detection of specific antibody-containing cells in the intestinal mucosa of mice |
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| Gao Jianxin,et al Wept.Microbiol.,Suzhou Medical College,Suzhou). A simplified immunofluorescence technique for detection of specific antibody-containing cells in the intestinal mucosa of mice[J]. Chinese Journal of Immunology, 1987, 0(4) |
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| Authors: | Gao Jianxin et al Wept.Microbiol. Suzhou Medical College Suzhou) |
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| Abstract: | It is an important means to detect specific antibody-containing cells(ACC)inthe intestinal mucosa by using immunofluorescent technique.The method of Pierceand of husband was modified by specific antisera in place of purified FITC-labelledantibody combining with corresponding antigen,which played a role of bridge bet-ween ACC and FITC-labelled non-specific antibody.The results demonstrated that themodified method was more simpler than Pierce and husband,and remained specificand sensitive)that soluble heat-stable antigen of CJ-S131 could be used as an antigenprobe for detection of specific ACC in the animal model in which killed CJ-S131 bac-teria was used as an immunogcn in the studies of mucosal immunity mechanisms. |
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| Keywords: | Immunofluorescent technique Mucosal immunity Heat-stable antigen antibody-containing cells Campylobacter jejuni. |
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