| HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量 |
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| 引用本文： | 魏悦,李晓,李自红,范毅,张海艳,赵天增. HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量[J]. 中国实验方剂学杂志, 2013, 19(9): 108-111 |
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| 作者姓名： | 魏悦 李晓 李自红 范毅 张海艳 赵天增 |
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| 作者单位： | 河南省生物技术开发中心,郑州,450002 |
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| 基金项目： | 河南省科技攻关项目(092102310016;112101310600) |
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| 摘 要： | 目的:建立连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量测定方法.方法:应用高效液相色谱-二极管阵列检测法(HPLC-DAD),根据各类成分紫外吸收光谱的差异,分别在354,329,277 nm波长下检测芦丁、连翘酯苷A和连翘苷.采用Sunfire C18(4.6 mm ×150 mm,5 μm)色谱柱,流动相为乙腈(A)-0.2％冰醋酸溶液(B),梯度洗脱(0 min,14％A;15min,18％ A;40 min,30％ A),流速1 mL· min-1,柱温40℃.结果:芦丁、连翘酯苷A和连翘苷分别在0.098～4.9,0.24 ～12.0,0.1 ～5.0 μg线性关系良好,平均回收率分别为98.63％,98.97％,102.18％,RSD分别为7.94％,0.93％,1.63％.结论:方法精密度高、重复性好,能够应用于连翘药材及提取物的质量控制.
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| 关 键 词： | 高效液相-二极管阵列检测法 芦丁 连翘酯苷 连翘苷 |
| 收稿时间： | 2012-11-20 |
Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC |
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| WEI Yue,LI Xiao,LI Zi-hong,FAN Yi,ZHANG Hai-yan and ZHAO Tian-zeng. Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(9): 108-111 |
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| Authors: | WEI Yue LI Xiao LI Zi-hong FAN Yi ZHANG Hai-yan ZHAO Tian-zeng |
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| Affiliation: | Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China;Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China;Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China;Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China;Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China;Biotechonology Developing Center of Henan Academy of Sciences, Zhengzhou 450002, China |
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| Abstract: | |
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| Keywords: | HPLC-DAD rutin forsythoside A phillyrin |
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