了哥王抗肿瘤活性部位筛选

目的:研究了哥王95%、75%、50%乙醇提取物、水提物及95%乙醇提取物的石油醚、氯仿、乙酸乙酯、正丁醇萃取部位对肿瘤细胞的增殖抑制作用,确定了哥王抗肿瘤活性部位。方法:采用四甲基偶氮唑盐(MTT)比色法考察了哥王不同提取物及95%乙醇提取物的不同溶剂萃取部位对Hela、SGC-7901、Bel-7402细胞的抗肿瘤活性。结果:了哥王95%乙醇提物对Hela、SGC-7901细胞有良好的抗肿瘤活性,其石油醚、氯仿、乙酸乙酯萃取部位对Hela、SGC-7901细胞均有不同程度的抗肿瘤活性。结论:石油醚、氯仿、乙酸乙酯萃取部位有较强的抗肿瘤活性,初步确定为了哥王抗肿瘤活性部位。

Preliminary Study on Anti-tumor Effect of Extracts of Wikstroemia indica (L.) C.A.Mey.

Objective:To estimate the anti-tumor activity of different extracts and different parts of 95% ethanol extracts of Wikstroemia indica (L.) C. A. Mey. Methods:MTT assay was applied to screen the anti-tumor effect of the extracts of Wikstroemia indica (L.) C. A. Mey. on 3 tumor cell lines in vitro.Results:95% ethanol extracts had strong inhibitory effect on different tumor cell lines.The anti-tumor activity of petroleum part, chloroform part and ethyl acetate part are various in vitro.Conclusion:Petroleum part, chloroform part and ethyl acetate part,which are considered as activity parts of Wikstroemia indica (L.) C. A. Mey.,have considerable cytotoxic effect.