蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系环氧合酶-2表达的影响研究

目的 探讨蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系环氧合酶-2 (COX-2)表达的影响.方法 用不同浓度的Agkihpin处理鼻咽癌CNE-2细胞系72 h后,应用免疫细胞化学、Western Blotting、反转录-聚合酶链反应(RT-PCR)法检测COX-2在CNE-2细胞系中的表达.结果 不同浓度Agkihpin处理CNE-2细胞系72 h后,COX-2的平均吸光密度比较,差异有统计学意义(P＜0.05);其中A1组、B1组和C1组较D1组均明显降低,差异有统计学意义(P＜0.05),A1组较B1组、C1组和D1组均明显降低,差异有统计学意义(P＜0.05).Western Blotting检测经不同浓度Agkihpin处理72 h后COX-2表达差异有统计学意义(P＜0.05),随着Agkihpin浓度的降低,COX-2表达水平升高,各组间比较差异均有统计学意义(P＜0.05).不同浓度Agkihpin处理72 h后COX-2 mRNA的相对转录水平比较差异有统计学意义(P＜0.05),随着Agkihpin浓度的降低,COX-2 mRNA的相对转录水平升高,各组间比较差异均有统计学意义(P＜0.05).结论 在人低分化鼻咽癌CNE-2细胞系中,Agkihpin能抑制COX-2的表达,并且随着浓度的增大更明显;Agkihpin对COX-2的抑制作用,可能成为鼻咽癌化疗的有效药物.

Impact of Agkihpin,An Arginine Esterase from Venom,on the Expression of COX-2 in Nasopharyngeal Carcinoma CNE-2 Cell Line

Objective To explore the impact of Agkihpin on the expression of cyclooxygenase-2 in nasopharyngeal carcinoma CNE-2 cell line and to describe the mechanisms of Agkihpin inhibition against CNE-2.Methods The cultured cells were treated with different concentrations of Agkihpin for 72 h.The expression levels of COX-2 in CNE-2 cell line were assayed by immunocytochemistry,Western blotting,and RT-PCR.Results The average COX-2 absorbance among different Agkihpin concentrations showed significant differences after...