| 氯霉素磁分离酶联免疫(MEIA)检测方法的建立 |
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| 引用本文： | Zhang B,Liu ZS,Yang H,Yang XL. 氯霉素磁分离酶联免疫(MEIA)检测方法的建立[J]. 细胞与分子免疫学杂志, 2011, 27(5): 585-6, 589 |
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| 作者姓名： | Zhang B Liu ZS Yang H Yang XL |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院,湖北,武汉,430074;北京倍爱康生物技术股份有限公司,北京,100070
2. 华中科技大学生命科学与技术学院,湖北,武汉,430074 |
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| 基金项目： | 武汉市科技攻关计划项目 |
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| 摘 要： | 目的:采用磁分离酶联免疫分析法(MEIA)建立定量检测氯霉素(CAP)的一种高灵敏度方法.方法:采用免疫竞争法,用异硫氰酸荧光素(FITC )标记抗CAP抗体,碱性磷酸酶(AP)标记CAP,以羊抗FITC抗体包被的免疫磁珠作为固相分离载体,单磷酸酚酞做显色底物,建立检测CAP的MEIA法.结果:成功建立了检测CAP的MEIA法,检测时间为40 min,检测灵敏度为0.03μg/L,线性范围为0.1～8.1μg/L,批内变异系数(CV) 3.9%-5.3%,批间CV 4.8%-8.1%,回收率为97%～101.5%,与国标方法液相色谱一串联质谱法的检测结果对比,相关系数r=0.9817.结论:检测CAP的MEIA法的灵敏度及准确率高、检测时间短,为食品中氯霉素残留的监测提供了一种新的免疫分析方法.
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| 关 键 词： | 氯霉素 磁分离酶联免疫,免疫检测 |
Methodological study on magnetic affinity immunoassay for detecting chloramphenicol |
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| Zhang Bo,Liu Zhen-shi,Yang Hai,Yang Xiang-liang. Methodological study on magnetic affinity immunoassay for detecting chloramphenicol[J]. Chinese journal of cellular and molecular immunology, 2011, 27(5): 585-6, 589 |
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| Authors: | Zhang Bo Liu Zhen-shi Yang Hai Yang Xiang-liang |
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| Affiliation: | ZHANG Bo1,2,LIU Zhen-shi1,YANG Hai1,YANG Xiang-liang1 1Biology Science and Technology College,Huazhong Science and Technology University,Wuhan 430074,2Beijing Bio-Ekon Biotechnology Co.Ltd,Beijing 100070,China |
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| Abstract: | AIM: To establish a sensitive immunoassay for detection chloramphenicol using magnetic beads as solid phase.METHODS: This assay employs the competitive inhibition,the FITC conjugated with anti-chloramphenicol monoclonal antibodies and the alkaline phosphatase conjugated with chloramphenicol respectively.Magnetic beads were coupled with sheep anti-FITC antibodies as solid phase.And phenolphthalein monophosphate was used as substrate to set up MEIA for detection chloramphenicol.RESULTS: MEIA for detection chl... |
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| Keywords: | chloramphenicol magnetic enzyme-linked immunoassay(MEIA) immunoassay |
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