| 检测肌糖蛋白C夹心ELISA方法的建立及初步应用 |
| |
| 作者姓名： | Wang YC Zhang DZ He LH Ding Y Shan LQ |
| |
| 作者单位： | 第四军医大学附属唐都医院骨科,陕西,西安,710038 |
| |
| 基金项目： | 国家自然科学基金资助项目 |
| |
| 摘 要： | 目的:以已经制备的抗肌糖蛋白C(TN-C)的单克隆抗体(mAb)为基础,建立能定量检测肌糖蛋白C浓度的夹心ELISA方法,并初步于临床血清标本检测.方法:将3株mAb(克隆号分别为:1A8、3H7和4D6)制备腹水后纯化,分别与辣根过氧化物酶交联后,两两配对,以重组肌糖蛋白C 蛋白为检测抗原,分析抗体之间最佳组合;利用建立的夹心ELISA方法检测收集的临床骨肉瘤患者和正常人血清标本.结果:包被1A8 mAb,HRP-4D6配对敏感性最高;骨肉瘤患者血清中肌糖蛋白C浓度明显高于正常人.结论:成功建立检测肌糖蛋白C的夹心ELISA方法,并利用其检测到肌糖蛋白C在骨肉瘤患者中的浓度异于正常人.
|
| 关 键 词： | 肌糖蛋白C 夹心ELISA 单克隆抗体 骨肉瘤 |
Establishment and primary application of sandwich ELISA method to detect tenascin-C |
| |
| Wang YC,Zhang DZ,He LH,Ding Y,Shan LQ.Establishment and primary application of sandwich ELISA method to detect tenascin-C[J].Journal of Cellular and Molecular Immunology,2011,27(6):694-696. |
| |
| Authors: | Wang Yu-cai Zhang Dian-Zhong He Li-hong Ding Yong Shan Le-qun |
| |
| Institution: | WANG Yu-cai,ZHANG Dian-zhong,HE Li-hong,DING Yong,SHAN Le-qun Department of Orthopaedics,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China |
| |
| Abstract: | AIM: To establish a sandwich method to detect tenascin-c on the basis of preparation of monoclonal antibodies(mAbs) against tenascin-C(TN-C).METHODS: The ascites of three stains of mAbs(No.1A8,3H7 and 4D6) were prepared and purified.The mAbs were conjugated with HRP and paired,respectively.The recombinant TN-C was taken as standard to analyze the optimal combination between mAbs.The sera TN-C concentrations of patients with osteosarcoma and the normal persons were evaluated with the sandwich ELISA method.RE... |
| |
| Keywords: | tenascin-C sandwich ELISA monoclonal antibody osteosarcoma |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
