林可霉素对树突状细胞系DC2.4免疫功能影响的初步研究

目的:探讨林可霉素(lin)对树突状细胞(DCs)系DC2.4免疫功能的影响。方法:设立3个组:DC2.4细胞组,DC2.4细胞+LPS组及DC2.4细胞+LPS+lin组(LPS及lin均为500 ng/mL)。在倒置显微镜下观察各实验组中DC2.4细胞的形态学变化。同时采用流式细胞仪分析DC2.4细胞表面标志MHC-Ⅱ类分子、CD86和CD80的表达。采用同种异体混合淋巴细胞反应(MLR)检测各实验组中DC2.4细胞刺激同种异体T淋巴细胞增殖的能力。用ELISA试剂盒检测干扰素-γ(IFN-γ)水平的变化。结果:倒置显微镜下显示,DC2.4细胞组为未成熟DCs形态,DC2.4细胞+LPS组及DC2.4细胞+LPS+lin两组均为成熟DCs的形态。流式细胞术分析证实,500 ng/mL LPS可以促进DC2.4细胞表面MHC-Ⅱ类分子、CD86和CD80的表达,并增强其刺激T细胞增殖及IFN-γ的分泌。但500 ng/mL lin联合500 ng/mL LPS能够部分抑制DC2.4细胞免疫调节作用。结论:Lin可以部分抑制成熟DC2.4细胞的免疫调节功能。

A preliminary study on the effect of lincomycin on the immune function of dendritic cell line DC2.4

AIM:To explore the effect of lincomycin(lin) on the immune function of dendritic cell(DCs) line DC2.4.METHODS:Three experimental groups,namely,DC2.4 cells group,DC2.4 Cells+LPS group and DC2.4 cells +LPS +lin group were established(LPS and lin were 500 ng/mL).The morphological changes in each group were observed under inverted microscope.The MHC class Ⅱ,CD86 and CD80 on the DC2.4 cells were detected by flow cytometer.The effects of lipopolysaccharide(LPS) and LPS +lin on the DC2.4 cells immuno-stimulatory capacity were evaluated by allogeneic mixed leukocytes reaction(MLR) between DC2.4 cells and T cells.ELISA was adopted to quantitate the level of IFN-γin the supernatant of cultured DC2.4 cells and T cells in each group.RESULTS:DC2.4 cells showed typical morphology of immature DCs.When stimulated with LPS or LPS combined with lin,DC2.4 cells exhibited typical maturate DCs modality.LPS of 500 ng/mL could significantly up-regulate the expression of MHC class Ⅱ,CD86 and CD80 on DC2.4 cells and also augment stimulatory action of DC2.4 cells on T cells proliferation and secretion of IFN-γ.However,compared with LPS alone,the treatment of lin(500 ng/mL) combined with LPS down-regulated the immmuno-regulation function of DC2.4 cells.CONCLUSION:Lin can partly inhibit the immuno-regulation function of maturate DC2.4 cells.