| 抗血小板膜糖蛋白Ib单克隆抗体的制备、鉴定及其功能的初步研究 |
| |
| 作者姓名： | Gu Y Ji SD Zhao YM Shen F Ruan CG |
| |
| 作者单位： | 苏州大学附属第一医院江苏省血液研究所,卫生部血栓与止血重点实验室,江苏,苏州,215006 |
| |
| 基金项目： | 国家自然科学基金资助项目,江苏省自然科学基金资助项目,江苏省高校自然科学基金资助项目 |
| |
| 摘 要： | 目的:制备抗人血小板膜糖蛋白Ib(GPIb)单克隆抗体(mAb),并进行生化鉴定与初步探讨其临床应用.方法:采用血小板免疫BALB/c小鼠,取其脾细胞与骨髓瘤细胞融合.经间接ELISA法筛选和克隆化培养,获得1株抗人GPIb mAb的杂交瘤细胞,纯化其腹水获得mAb;免疫双扩散鉴定其抗体亚类;流式细胞术和免疫放射法鉴定...
|
| 关 键 词： | 血小板 糖蛋白Ib 单克隆抗体 血管性血友病因子 |
Development, identification and function assay of monoclonal antibody against platelet membrane glycoprotein Ib |
| |
| Gu Y,Ji SD,Zhao YM,Shen F,Ruan CG.Development, identification and function assay of monoclonal antibody against platelet membrane glycoprotein Ib[J].Journal of Cellular and Molecular Immunology,2011,27(6):650-652. |
| |
| Authors: | Gu Ying Ji Shun-dong Zhao Yi-ming Shen Fei Ruan Chang-geng |
| |
| Institution: | GU Ying,JI Shun-dong,ZHAO Yi-ming,SHEN Fei,RUAN Chang-geng Jiangsu Institute of Hematology,The First Affiliated Hospital of Soochow University,Key Laboratory of Thrombosis and Hemostasis,Ministry of Health,Suzhou 215006,China |
| |
| Abstract: | AIM: To prepare and identify a monoclonal antibody(mAb) against human platelet glycoprotein Ib and make its application.METHODS: BALB/c mice were immunized with human platelets washed,and the spleen cells of them were fused with myloma cells.A hybridoma cell was screened by indirect ELISA and cloned,and the mAb were purified from the ascites of mice.Ig subclass was analysed by double immunodiffusion.The antigen recognized by monoclonal antibody was identified by flow cytometry and radioimmunoassy,respective... |
| |
| Keywords: | platelet glycoprotein Ib monoclonal antibody von Willebrand factor |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
