| 雷帕霉素对人外周血T淋巴细胞上BTLA表达的影响 |
| |
| 作者姓名： | Ding CG Tian PX Yan H Xue WJ Ge GQ Jin ZK Li Y |
| |
| 作者单位： | 西安交通大学医学院第一附属医院肾病中心肾移植科,陕西,西安,710061 |
| |
| 基金项目： | 国家自然科学基金资助项目,陕西省科技攻关项目基金资助,西安市科技计划资助项目,陕西省自然科学基金资助,陕西13115重大科技攻关资助项目 |
| |
| 摘 要： | 目的:探讨雷帕霉素(rapamycin,RPM)对人外周血T淋巴细胞上B、T淋巴细胞弱化子(BTLA)表达的影响,为其在器官移植和自身免疫性疾病中的应用提供实验依据。方法:采用Ficoll密度梯度离心法分离人外周血单核细胞(PBMCs),然后利用免疫磁珠分离技术分离PBMCs中的T细胞。用刀豆蛋白A刺激、活化人外周血T细胞。用MTT比色法测定T细胞的增殖。用ELISA法测定细胞培养上清液中IL-2、IFN-γ的水平。用流式细胞术检测T细胞上BTLA的表达。结果:用不同浓度(10~1 000 mL)的RPM处理的T淋巴细胞上BTLA的表达无显著差别;而与未经RPM处理组比较差别显著(P<0.01)。ELISA检测结果显示不同浓度的RPM与未处理组相比均能显著抑制IL-2和IFN-γ的分泌(P<0.01),并可以剂量依赖的方式显著抑制T淋巴细胞增殖(P<0.01)。结论:RPM对BTLA的表达影响较小,而对T淋巴细胞增殖和IL-2、IFN-γ的分泌有较强的抑制作用,提示RPM适宜用于器官移植排斥反应和自身免疫性疾病的治疗。
|
| 关 键 词： | 雷帕霉素 BTLA T细胞 排斥反应 自身免疫病 |
The influence of rapamycin on BTLA expression of human peripheral blood T lymphocytes |
| |
| Ding CG,Tian PX,Yan H,Xue WJ,Ge GQ,Jin ZK,Li Y.The influence of rapamycin on BTLA expression of human peripheral blood T lymphocytes[J].Journal of Cellular and Molecular Immunology,2011,27(7):751-753. |
| |
| Authors: | Ding Chen-guang Tian Pu-xun Yan Hang Xue Wu-jun Ge Guan-qun Jin Zhan-kui Li Yang |
| |
| Institution: | DING Chen-guang,TIAN Pu-xun,YAN Hang,XUE Wu-jun,GE Guan-qun,JIN Zhan-kui,LI YangDepartment of Renal Transplantation,Center of Nephropathy,the First Affiliated Hospital,Medical College of Xi'an Jiaotong University,Xi'an 710061,China |
| |
| Abstract: | AIM:To explore the effect of rapamycin(RPM) on the expression of B and T lymphocyte attenuate(BTLA) on human peripheral blood T lymphocytes,providing a experimental basis for application of RPM to organ transplantation and autoimmune diseases.METHODS:Human peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation,and then the T cells were isolated from PBMCs with immunomagnetic beads.Concanavalin A(Con A) used to stimulate and activate the human peripheral blood T cells.The ... |
| |
| Keywords: | BTLA |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
