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| 胃癌中REIC蛋白分泌水平及其增殖抑制作用研究 | |
| 作者姓名： | Xu XY Xia P Xing YN Zhang DH Takano Y Zheng HC |
| 作者单位： | 1. 中国医科大学基础医学院病理生理学教研室,辽宁,沈阳,110001 2. 中国医科大学基础医学院病理学与病理生理学研究所,辽宁,沈阳,110001 3. 中国医科大学附属第一医院肿瘤外科,辽宁,沈阳,110001 4. 大连医科大学口腔医学院09级2班,辽宁,大连,116044 5. 日本神奈川县癌中心临床研究所,日本,241-0815 |
| 基金项目： | 国家自然科学基金青年科学墓金项目,辽宁省教育厅科学技术研究项目 |
| 摘 要： | 目的:研究胃癌患者血清和胃癌细胞培养液上清中分泌型REIC含量及重组REIC对胃癌细胞增殖的影响.方法:ELISA检测血清和细胞上清中的REIC表达;WST-8法检测胃癌细胞的增殖.结果:REIC在胃癌患者中的分泌水平比正常健康人明显降低(P<0.05),并且与肿块大小呈负相关(P<0.05);胃癌细胞系培养液上清中的REIC表达低于正常的胃勃膜细胞(P<0.05);胃癌AGS细胞系转染REIC后,细胞培养液上清中的REIC浓度升高(P<0.05);加人外源性融合的REIC可以抑制胃癌细胞AGS和BGC-823的增殖(P<0.05).结论:分泌型的REIC在胃癌细胞中表达降低,重组REIC蛋白可抑制胃癌细胞的增殖,REIC蛋白可以作为胃癌早诊、生物学和基因治疗的靶点. |
| 关 键 词： | 胃癌 血清 |
The secreted level of REIC protein in gastric carcinoma and its role of proliferation inhibition |
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| Xu XY,Xia P,Xing YN,Zhang DH,Takano Y,Zheng HC.The secreted level of REIC protein in gastric carcinoma and its role of proliferation inhibition[J].Journal of Cellular and Molecular Immunology,2011,27(5):568-570. | |
| Authors: | Xu Xiao-yan Xia Pu Xing Ya-nan Zhang Dong-han Takano Yasuo Zheng Hua-chuan |
| Institution: | XU Xiao-yan1,XIA Pu2,XING Ya-nan3,ZHANG Dong-han4,TAKANO Yasuo5,ZHENG Hua-chuan2 1Department of Pathophysiology,College of Basic Medicine,China Medical University,Shenyang 110001,2Institute of Pathology and Pathophysiology,3Department of Surgical Oncology,the First Affiliated Hospital,4Grade 09 Class 2,College of Stomatology,Dalian Medical University,Dalian 116044,China,5Clinical Research Institute,Ka... |
| Abstract: | AIM: To detect secretory REIC content in serum from the patients with gastric carcinoma and cell culture medium of gastric carcinoma cell lines,and determine the effects of recombinant REIC on proliferation of gastric carcinoma cells.METHODS: ELISA was employed to determine REIC content in serum and supernatant,and WST-8 to detect the proliferation of gastric carcinoma cells.RESULTS: The secretion level of REIC was obviously decreased in serum form gastric carcinoma,compared with healthy individuals(P<0.05)... |
| Keywords: | REIC ELISA |
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