莪术醇对鼻咽癌细胞CEN-2增殖与凋亡的影响

目的:观察莪术醇对鼻咽癌CEN-2的增殖抑制与凋亡的影响,探讨其抗鼻咽癌的分子机制。方法:以不同浓度的莪术醇(12.5、25、50、100 mg/L)及阴性对照组处理鼻咽癌CNE-2细胞,应用MTT法检测不同浓度的莪术醇对CNE-2细胞生长增殖抑制作用;Hochest33342荧光染色法观察凋亡细胞的发生及形态学变化,流式细胞术检测其凋亡率,Western blot法检测核转录因子-κB(NF-κB)蛋白的表达。结果:莪术醇处理组能明显抑制CNE-2的增殖(P<0.01),流式细胞术检测药物作用48 h后,CNE-2细胞的凋亡率也随莪术醇浓度的增加而增加,100 mg/L的莪术醇处理组其凋亡率可达45.5%,与阴性组比差异极显著(P<0.01);NF-κB的蛋白相对表达量随莪术醇剂量的增大而下降,与对照组相比有显著性意义(P<0.01)。结论:莪术醇能对体外鼻咽癌CNE-2细胞具有明显增殖抑制和诱导凋亡的作用,其分子机制可能与NF-κB的表达下调有关。

Effect of curcumol on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2

AIM:To investigate the effect of curcumol on the proliferation,apoptosis and the expression NF-κB in nasopharyngeal carcinoma cell line CNE-2.METHODS:CNE-2 cells were treated with curcumol at different concentration(12.5,25,50,100 mg/L) and the control group;the effect of proliferation was detected by MTT method;the apoptosis was analyzed by hoechst 33342 flourescence staning and flow cytometry;the expression of NF-κB was detected with western blotting.RESULTS:After treated with cucrcumol,CNE-2 cell’s proliferation was significantly reduced(P<0.01) and its apoptosis was increased as the curcumol concentration rising,the apoptosis of 100 mg/L curcumol group even can rearch to 45.5% and it was a significantly difference compared with control group(P<0.01);the expression of NF-κB was down regulated as raising the curcumol concentration,there was a significantly difference compared with control group(P<0.01).CONCLUSION:Curcumol is capable of significantly inhibitiing proliferation and inducing apoptosis of CNE-2’ cells in vitro,the mechanism of curcumol anti-tumor may be related to the down regulated of the NF-κB protein level.