| 索拉非尼抑制肝癌细胞增殖中自噬的作用及其机制 |
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| 引用本文： | 杜海磊,杨卫平,陈聆,施敏敏,汪洋,李军,刘卓然,陈拥军,严佶祺,匡洁,李勤裕,邱伟华,Seewoo Varuna.索拉非尼抑制肝癌细胞增殖中自噬的作用及其机制[J].外科理论与实践,2011,16(3):270-274. |
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| 作者姓名： | 杜海磊 杨卫平 陈聆 施敏敏 汪洋 李军 刘卓然 陈拥军 严佶祺 匡洁 李勤裕 邱伟华 Seewoo Varuna |
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| 作者单位： | 1. 上海交通大学医学院附属瑞金医院,外科,上海,200025
2. 上海交通大学医学院附属瑞金医院,上海消化外科研究所,上海,200025 |
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| 基金项目： | 国家自然科学基金，上海市自然科学基金，上海市慈善癌症研究基金 |
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| 摘 要： | 目的：研究分子靶向药物索拉非尼在体外对人肝癌细胞株HepG_2增殖抑制过程中自噬的表达及作用,并探讨其可能机制。方法：以吖啶橙染色荧光显微镜对自噬进行定性观察;cell counting kit-8检测活性氧（reactiveoxygen species,ROS）抑制前后HepG_2细胞成活率的变化;RT-PCR检测自噬基因Beclin-1表达的变化。Western印迹检测自噬相关蛋白Beclin-1的变化：荧光分光光度计检测胞内二氯荧光素DCF的荧光强度。结果：索拉非尼对肝癌细胞HepG_2具有显著的抑制作用;索拉非尼可诱导肝癌细胞HepG_2产生自噬及ROS,自噬在基因及蛋白水平表达均增加;抑制ROS的产生可减少索拉非尼诱导的肝癌细胞HepG_2自噬的表达量,自噬的抑制增强了索拉非尼对肝癌细胞的抑制作用。结论：ROS参与索拉非尼诱导肝癌细胞HepG_2的自噬表达,索拉非尼在抑制肝癌细胞自噬过程中自噬可能起到保护作用,抑制自噬可能为提高进展期肝癌病人索拉非尼分子靶向治疗敏感性提供新的思路。
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| 关 键 词： | 肝癌 自噬 索拉非尼 活性氧 |
Effect and mechanism of sorafenib in inhibiting autophagy of HepG2 cells proliferation |
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| DU Hai-lei,YANG Weiping,CHEN Ling,SHI Min-min,WANG Yang,LI Jun,LIU Zhuo-ran,CHEN Yong-jun,YAN Ji-qia,KUANG Jie,LI Qin-yu,SEEWOO Varuna,Seewoo Varuna.Effect and mechanism of sorafenib in inhibiting autophagy of HepG2 cells proliferation[J].Journal of Surgery Concepts & Practice,2011,16(3):270-274. |
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| Authors: | DU Hai-lei YANG Weiping CHEN Ling SHI Min-min WANG Yang LI Jun LIU Zhuo-ran CHEN Yong-jun YAN Ji-qia KUANG Jie LI Qin-yu SEEWOO Varuna Seewoo Varuna |
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| Institution: | DU Hai-lei~a,YANG Wei-ping~a,CHEN Ling~a,SHI Min-min~b,WANG Yang~a,LI Jun~a,LIU Zhuo-ran~a,CHEN Yong-jun~a,YAN Ji-qi~a,KUANG Jie~a,LI Qin-yu~a,SEEWOO Varun~a,QIU Wei-hua~a.a.Department of Surgery,b.Shanghai Institute of Digestive Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China. |
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| Abstract: | Objective This study was designed to investigate the effects and mechanism of autophagy on the viability change of human hepatoma cell line HepG_2 induced by sorafenib.Methods The acidic vesicular organelles were observed using fluorescent microscope by acriding orange staining.The viability change of the HepG_2 cell line induced by sorafenib was analyzed by using cell counting kit-8 in the presence or absence of reactive oxygen species(ROS) scavenger. RT-PCR and Western blot were used to examine Beclin-1(a... |
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| Keywords: | Hepatocellular carcinoma Autophagy Sorafenib Reactive oxygen species |
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