| 脂多糖刺激下大鼠腹膜间质细胞IL-18、IL-6的表达和氧化应激的影响 |
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| 作者姓名： | Li CC Ma JF Peng X Chen S Zhang XL |
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| 作者单位： | 中国医科大学附属第一临床医院肾内科,辽宁,沈阳,110001 |
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| 摘 要： | 目的:研究脂多糖刺激下大鼠腹膜间皮细胞(RPMC)IL-18、IL-6和氧化应激产物丙二醛(MDA)的表达。方法:原代培养RPMC,用不同浓度脂多糖(1、10、100 mg/L)刺激RPMC 6 h;10 mg/L脂多糖刺激RPMC 3、6、12、24 h。用real time-PCR法检测IL-18mRNA的表达,ELISA法检测细胞上清液中IL-18和IL-6的蛋白水平,硫代巴比妥酸法检测细胞中MDA的含量。结果:与正常对照组比较,脂多糖刺激下RPMC IL-18、IL-6和MDA的表达明显增加(P<0.05),且呈浓度依赖性;随着刺激时间的延长,上述指标呈递增趋势,IL-18于12 h达高峰。结论:脂多糖刺激下RPMC促炎症因子IL-18、炎症因子IL-6及氧化应激产物MDA的表达均增加,引起持续放大的炎症氧化反应,损伤腹膜导致超滤失败。
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| 关 键 词： | 脂多糖 腹膜间皮细胞 IL-18 IL-6 丙二醛 |
Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide |
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| Li CC,Ma JF,Peng X,Chen S,Zhang XL.Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide[J].Journal of Cellular and Molecular Immunology,2011,27(6):688-690. |
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| Authors: | Li Cheng-cheng Ma Jian-fei Peng Xia Chen Shuo Zhang Xiu-li |
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| Institution: | LI Cheng-cheng,MA Jian-fei,PENG Xia,CHEN Shuo,ZHANG Xiu-li Department of Nephrology,First Affiliated Hospital,China Medical University,Shenyang 110001,China |
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| Abstract: | AIM: To observe the expression of IL-18,IL-6 and the production of oxidative stress Malondialdehyde(MDA)in rat peritoneal mesothelial cells(RPMC) stimulated by Lipopolysaccharide(LPS).METHODS: RPMC were primordially culture and stimu lated with different concentrations(1,10,100 mg/L) LPS for 6h;RPMC were stimulated by 10 mg/L LPS for 3,6,12,24 h.IL-18 mRNA was detected by real time-PCR.IL-6 and IL-18 were detected by ELISA in supernatants.MDA was measured by thiobarbituric acid method.RESULTS: Compared with... |
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| Keywords: | IL-18 IL-6 |
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