纳米磷酸钙对MG63细胞生物学行为的影响

背景:纳米材料的尺寸、形貌、结晶度等性质对其毒性具有明显的影响。目的:选用3种不同形貌的磷酸钙纳米颗粒来探索材料形貌对MG63细胞各种生物学行为的影响,并分析其物理化学性质与生物学效应之间的关系。方法:利用水热法和化学沉淀法制备球形、梭形和棒状3种不同形貌的磷酸钙颗粒并铺膜,通过透射电镜、场发射扫描电镜、原子力显微镜、动态光散射、X射线衍射对磷酸钙颗粒及其膜的尺寸、形貌、粗糙度和结晶度等性质进行表征。在磷酸钙膜上培养MG63细胞,检测磷酸钙对细胞的黏附、增殖、分化及凋亡的影响。结果与结论:磷酸钙形貌及结晶度对MG63细胞的增殖具有明显的影响。颗粒长径比大,结晶度高的颗粒,能促进细胞的增殖;反之,则抑制细胞的增殖。磷酸钙膜的粗糙度对细胞的黏附过程具有明显的影响,粗糙度越大,抑制作用越明显。3种磷酸钙对细胞的分化和凋亡没有明显的影响。

Effect of calcium phosphate nanoparticles on biological behaviors of MG63 cells

BACKGROUND:Several studies have shown that many features of nanomaterials can influence nanomaterials toxicity obviously, such as their size, shape and crystallinity. OBJECTIVE:To explore the effect of material shapes on the biological behaviors of MG63 cells and to analyze the correlations between their physical-chemical properties and biological effect by using three kinds of calcium phosphate nanoparticles with different shapes. METHODS:Spherical, fusiform and rod-like calcium phosphate nanoparticles were synthesized by hydro-thermal method and chemical precipitation method and filmed on the glasses. The size, shape, roughness and crystallinity of calcium phosphate nanoparticles and their films were characterized by transmission electron microscope, field emission scanning electron microscopy, atomic force microscopy, dynamic light scattering and X-ray diffraction. MG63 cells were seeded and cultured on films. Effects of calcium phosphate on the biological behaviors of MG63 were detected, including adhesion, proliferation, differentiation, and apoptosis. RESULTS AND CONCLUSION:The results showed that the shape and crystallinity of calcium phosphate influenced MG63 cells proliferation obviously. Calcium phosphate nanoparticles with high aspect ratio and high crystallinity could promote cells proliferation, and conversely, calcium phosphate nanoparticles with low aspect ration and low crystallinity could inhibit cells proliferation. MG63 cells were sensitive to the roughness of calcium phosphate films, and cell adhesion ability was decreased with the increase of the roughness of calcium phosphate films. These findings suggest that the three kinds of calcium phosphate can promote cells migration effectively, but there are no obvious influences on cells differentiation and apoptosis.