乌司他丁联合脐带间充质干细胞移植脊髓损伤大鼠后肢运动功能和运动诱发电位的变化

背景:乌司他丁能减轻炎性反应、清除氧自由基,对中枢神经系统损伤具有保护作用,能有效地提高脊髓损伤后移植细胞的存活率。目的:观察乌司他丁联合脐带间充质干细胞移植对脊髓损伤大鼠后肢功能的影响。方法:Wistar大鼠建立脊髓损伤动物模型后随机分成4组:空白对照组尾静脉注射培养液+腹腔注射生理盐水,细胞移植组尾静脉注射脐带间充质干细胞,乌司他丁组腹腔注入乌司他丁,联合组尾静脉注射脐带间充质干细胞,同时腹腔注入乌司他丁。结果与结论:移植4周后联合组下肢运动功能优于细胞移植组和乌司他丁组(P<0.05),细胞移植组和乌司他丁组优于空白对照组(P<0.05)。移植后4周,PKH26标记的阳性细胞数联合移植组多于细胞移植组,细胞移植组多于乌司他丁组和空白对照组(P<0.01)。移植后8周,联合组大鼠体感诱发电位及运动诱发电位的潜伏期、波幅明显优于其他3组(P<0.05或P<0.01)。提示乌司他丁联合应用脐带间充质干细胞移植可促进脊髓损伤大鼠神经突触的再生,改善其肢体运动功能和电生理功能,其效果优于单独应用乌司他丁或脐带间充质干细胞。

Effects of ulinastatin application combined with umbilical cord mesenchymal stem cells transplantation on changes in motor evoked potential and motor function of hindlimbs in rats with spinal cord injury

BACKGROUND:Ulinastatin can reduce the inflammatory response,and get rid of oxygen free radicals.Ulinastatin has a protective effect on the central nervous system injury,and can effectively increase the survival rate of transplanted cells after spinal cord injury.OBJECTIVE:To investigate the effects of umbilical cord mesenchymal stem cells transplantation and ulinastatin on the repair of motor function of hindlimbs in rats with spinal cord injury.METHODS:Wistar rats were used to establish the spinal cord injury models and the models were randomly divided into four groups:control group,umbilical cord mesenchymal stem cells group,ulinastatin group and combination group.The models in the control group underwent tail vein injection of culture medium and intraperitoneal injection of normal saline.The umbilical cord mesenchymal stem cells group was injected with umbilical cord-matrix stem cells via the tail vein.The ulinastatin group underwent intraperitoneal injection of ulinastatin and the combination group underwent tail vein injection of umbilical cord mesenchymal stem cells and intraperitoneal injection of ulinastatin.RESULTS AND CONCLUSION:At 4 weeks after transplantation,the function of rat hindlimbs in the combination group was superior to that in the umbilical cord mesenchymal stem cells transplantation group and the ulinastatin group (P < 0.05),and the hindlimb function was superior in the umbilical cord mesenchymal stem cells transplantation group and the ulinastatin group to that in the control group (P < 0.05).The number of PKH26-positive cells in the combination group was greater than that in the umbilical cord mesenchymal stem cells transplantation group,and the number was also significantly greater in the umbilical cord mesenchymal stem cells transplantation group than that in the ulinastatin group and control group (P < 0.01).At 8 weeks after transplantation,the latency and amplitude of somatosensory evoked potential and motor evoked potential in the combination group were significantly better than those in the other three groups (P < 0.05,P < 0.01).Umbilical cord mesenchymal stem cells transplantation combined with the ulinastatin can promote the regeneration of nerve synapses in rats with spinal cord injury,and can improve their motor function and electrophysiological function.The umbilical cord mesenchymal stem cells transplantation combined with the ulinastatin produces better effects than single use in repair of motor function of hundlimbs of rats with spinal cord injury.