成人再生障碍性贫血患者骨髓间充质干细胞增殖分化潜能:与健康人的比较

背景:骨髓间充质干细胞具有成脂和成骨分化潜能,再生障碍性贫血患者骨髓间充质干细胞可能存在成脂和成骨分化异常。目的:观察再生障碍性贫血患者骨髓间充质干细胞增殖及分化潜能与健康人的差异。方法:选择哈尔滨市第一医院血液病肿瘤研究所收治的再生障碍性贫血患者5例,另选5例健康成人作为对照组;采用全骨髓培养法分离骨髓间充质干细胞。结果与结论:采用全骨髓培养法成功分离出骨髓间充质干细胞;与对照组相比,再生障碍性贫血组骨髓间充质干细胞的细胞形态和免疫表型无显著差异,皆表达CD73、CD90、CD105,不表达CD34、CD45和HLA-DR;再生障碍性贫血组骨髓间充质干细胞增殖能力显著低于对照组(P<0.05),成脂肪细胞分化诱导率高于对照组(P<0.05),PPARγmRNA和FABP4 mRNA表达量均高于对照组(P<0.05);而成骨细胞分化诱导率低于正常对照组(P<0.05),ALPmRNA和BGLAP mRNA表达量分别低于对照组(P<0.05)。提示再生障碍性贫血组骨髓间充质干细胞分化潜能存在成脂肪细胞和成骨细胞分化的失衡。

Proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells in adult patients with aplastic anemia versus in healthy adults

BACKGROUND：Bone marrow-derived mesenchymal stem cells （BMSCs） have adipogenic and osteogenic differentiation potential. BMSCs in patients with aplastic anemia （AA） may suffer from adipogenic and osteogenic differentiation abnormality. OBJECTIVE：To compare the proliferation and differentiation potential of BMSCs in AA patients versus in healthy adults. METHODS：AA group included five adult patients who received treatment in the Institute of Hematology Oncology, Harbin First Hospital. Control group consisted of five healthy adults. BMSCs were isolated by whole bone marrow culture method. RESULTS AND CONCLUSION：BMSCs were isolated and purified successfully and effectively. Morphology and immunophenotype of BMSCs from AA group showed no significant difference compared with control group. Two groups were all positive for MSC-related antigens, such as CD73, CD90, CD105, but negative for CD34, CD45, and HLA-DR. Compared with the control group, BMSC proliferation was significantly lower （P 0.05）, the frequency of adipocyte differentiation was significantly higher （P 0.05）, PPARγ mRNA and FABP4 mRNA expression was significantly higher （P 0.05）, the frequency of osteoblast differentiation was significantly lower （P 0.05）, and ALP mRNA and BGLAP mRNA expression was significantly decreased （P 0.05） in the AA group. These findings suggest that BMSCs from AA patients exhibit unbalanced differentiation potential of osteogenesis and adipocyte.