不同性别小鼠化学性肝损伤后的肝再生能力比较

背景:肝脏再生能力受多重因素的影响,而性别是否是再生修复过程的一个重要因素尚无定论。目的:比较不同性别小鼠化学性肝损伤后肝脏再生能力是否有差异。方法:采用四氯化碳小鼠皮下注射方法建立急性化学性肝损伤模型。120只昆明小鼠随机均分为糖原染色组和5-溴脱氧尿嘧啶核苷组,各组再按照性别均分为雌雄两组,于造模后第1,3,5,7,10天完整取下小鼠肝脏并称质量,取材前称量小鼠体质量。结果与结论:造模后第7天雌性鼠恢复原肝质量/体质量比,而雄性鼠则表现为下降趋势,不同性别小鼠肝质量/体质量比在各时间点差异无显著性意义(P>0.05);四氯化碳诱导的急性肝损伤主要部位为肝小叶周围的门管区及界板区,损伤部位肝细胞出现脂肪变,少数肝组织出现点状坏死;损伤后1,5,7d雌性小鼠糖原染色明显强于雄性组(P<0.05);肝损伤后3d雌性鼠肝脏5-溴脱氧尿嘧啶核苷标记指数高于雄性小鼠(P<0.05),而第1,5,7,10天则未见明显差异(P>0.05)。说明肝损伤后雌性鼠的再生修复比雄性鼠强,于第7天完成修复。不同性别的昆明小鼠对四氯化碳诱导的急性肝损伤后恢复期中肝脏再生能力具有差异性。

Comparison of hepatic regenerative capacity between male and female mice after chemical hepatic injuries

BACKGROUND:Many factors can influence the regeneration ability of liver.However,whether the effect of gender is one of the important factors during the liver repairing is uncertain.OBJECTIVE:To compare the hepatic regenerative capacity between male and female mice after chemical injuries.METHODS:Mouse models of acute chemical liver injury were established by carbon tetrachloride subcutaneous injection.120 mice were divided into two groups:glycogen staining group and 5-bromodeoxyuridine group,60 mice in each group,half male and half female.The intact liver was removed to be weighed on 1,3,5,7 and 10 days.The mice were weighed before operation.RESULTS AND CONCLUSION:The liver/body mass ratio in female mice was recovered at 7 days,but the ratio of the male mice was decreased.There was no distinct difference in the liver/body mass ratio between different genders at different time points (P > 0.05).The main injured sites of the liver induced by carbon tetrachloride was portal area and board area around the hepatic lobule,the liver cells presented with fatty degeneration,and the spotty necrosis was seen in the liver tissue.The female mice glycogen staining was darker than male group at 1,5 and 7 days (P < 0.05).The liver 5-bromodeoxyuridine labeling index of female mice was higher than that of male mice at 3 days after liver injury (P < 0.05).There was no difference at 1,5 and 7 days (P > 0.05).As a result,the recover ability after liver injury of female mice is more obvious than that of male mice,especially at 7 days,so different gender mice has different recover ability after liver injury.