兔角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达与高压氧的影响

背景:血管内皮生长因子和肿瘤坏死因子α在角膜移植免疫反应中起重要作用,但二者与高压氧之间的关系尚不明确。目的:探讨高压氧对角膜移植后房水中血管内皮生长因子及肿瘤坏死因子α表达的影响及其与角膜移植免疫反应的关系。方法:分别以鸡、兔为供、受体建立异种异体穿透性角膜移植模型。将健康新西兰白兔16只随机分为2组,高压氧组从手术当日至术后第6天行高压氧治疗,对照组未行高压氧治疗。分别于角膜移植后第3,7,14,28天抽取房水,应用ELISA双抗体夹心法检测房水中肿瘤坏死因子α及血管内皮生长因子水平的变化。结果与结论:正常兔眼房水中均可检测到少量的肿瘤坏死因子α和血管内皮生长因子。移植后早期两组房水中肿瘤坏死因子α和血管内皮生长因子水平即升高,与移植前比差异均有显著性意义(P<0.05)。在移植后14d左右急性排斥反应期,肿瘤坏死因子α和血管内皮生长因子达最高值。高压氧组中肿瘤坏死因子α和血管内皮生长因子的水平均明显低于对照组(P<0.05)。结果表明高压氧可通过降低房水中肿瘤坏死因子α和血管内皮生长因子的含量,抑制角膜移植后的免疫反应。

Effects of hyperbaric oxygen on the expression of vascular endothelial growth factor and tumor necrosis factor alpha in the aqueous humour after penetrating keratoplasty in rabbits

BACKGROUND:Studies had demonstrated that vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α) may play an important role in immunologic rejection after penetrating keratoplasty,but the interactions between hyperbaric oxygen,VEGF and TNF-α remains unclear.OBJECTIVE:To investigate the effects of hyperbaric oxygen on the expression of VEGF and TNF-α in the aqueous humour after penetrating keratoplasty,and to study the relationship between VEGF,TNF-α and cornea transplantation immunologic rejection.METHODS:We used the chicken to rabbit model of penetrating heterograft.Sixteen healthy New Zealand rabbits were randomly divided into two groups:control group and hyperbaric oxygen group.The rabbits in the hyperbaric oxygen group were treated with hyperbaric oxygen from the 1 st day to the 6 th day after operation,and the rabbits in the control group were not treated with hyperbaric oxygen.The level of VEGF and TNF-α were determined dynamically by enzyme linked immunosorbent assay on 3,7,14,and 28 days after surgery.RESULTS AND CONCLUSION:The TNF-α and VEGF expression could be seen in the aqueous humour of normal rabbits,and the levels were increased in early postoperative days,which had a significant difference statistically before and after surgery (P < 0.05).In the acute rejection period,at 14 days after transplantation,the quantity of TNF-α and VEGF reached their peak respectively,The content of TNF-α and VEGF in the hyperbaric oxygen group were obviously lower than those in the control group (P < 0.05).Hyperbaric oxygen can effectively reduce the level of VEGF and TNF-α and inhibit the immune response after keratoplasty.