生物素多聚葡聚糖胺追踪显示皮质脊髓束的走行和分布

背景:生物素多聚葡聚糖胺的作用机制及代谢过程目前还不十分清楚,可能和与生物素连接的其他复合物相似。目的:选择最佳的生物素多聚葡聚糖胺追踪(示踪)实验方法,使生物素多聚葡聚糖胺能充分显示皮质脊髓束的走行和分布。方法:将15只SD雌性大鼠用于生物素多聚葡聚糖胺条件实验,分为双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5μL/位点存活2周组、单侧(右侧)大脑皮质注射10%生物素多聚葡聚糖胺1μL/位点存活3周组及双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5μL/位点存活3周组,每组5只。结果与结论:在3组进行生物素多聚葡聚糖胺追踪实验的大鼠中,双侧大脑皮质注射10%生物素多聚葡聚糖胺0.5μL/位点存活3周组的追踪效果最好。提示在双侧大脑皮质上选择多点注射,以每个位点注射0.5μL的10%生物素多聚葡聚糖胺,注射后大鼠存活3周为最佳的生物素多聚葡聚糖胺追踪实验方案。

Demonstration empirical study of the corticospinal tracts by biotinylated dextran amine tracer

BACKGROUND:Biotinylated dextran amine mechanisms and metabolic processes are not entirely clear,which may be similar to the complex linked with biotin.OBJECTIVE:To choose the best biotinylated dextran amine tracing empirical method to fully show courser and distribution of the corticospinal tracts METHODS:Fifteen normal Sprague-Dawley female rats were used in this study and divided into three groups,with five rats in each group:in the first group,rats survived for 2 weeks after 10% biotinylated dextran amine(0.5 μL/site) was injected into the bilateral cerebral cortices;while 10% biotinylated dextran amine(1 μL/site) was injected into the right cerebral cortex in the second group rats which survived for 3 weeks;in the third group,10% biotinylated dextran amine(0.5 μL/site) was injected into the bilateral cerebral cortices,and the rats survived for 3 weeks.RESULTS AND CONCLUSION:The trace effect of biotinylated dextran amine in the third group was the best.This indicates that the tracing effect of biotinylated dextran amine is optimal in the rats receiving multi-point injection of biotinylated dextran amine(10%,0.5 μL/site) into the bilateral cerebral cortices and surviving for 3 weeks after injection.