2型糖尿病非收缩性难愈伤口模型的建立

背景:目前糖尿病创口的难愈性给临床的治疗增加了极大地困难。目的:建立2型糖尿病状态下非收缩性难愈伤口的动物模型。方法:高脂饮食喂养联合低剂量链脲佐菌素诱导形成大鼠2型糖尿病模型,在其背部造成圆形创面,用硅树脂固定伤口,模拟人类创面愈合过程,病理切片检查创面周缘肉芽组织皮肤厚度、微血管密度、胶原纤维及胶原蛋白水平的变化。结果与结论:与正常组相比,链脲佐菌素注射72h后高脂饮食喂养大鼠的空腹血糖、血清三酰甘油及胆固醇均明显增加,差异有显著性意义(P<0.05～0.01);创伤后第1,3,5,7,14天,糖尿病组与正常组相比皮肤厚度、微血管密度、胶原纤维及胶原蛋白水平均有明显下降(P<0.01)。提示高脂喂养联合链脲佐菌素诱导大鼠2型糖尿病的方法简便有效;创伤后检测指标均证明糖尿病大鼠比正常大鼠创面难愈。

Establishment of a non-contractile refractory wound model in type 2 diabetic rats

BACKGROUND:Currently,refractory diabetic wounds have brought significant difficulties in clinical treatment of diabetic patients.OBJECTIVE:To establish a model of non-contractile refractory wound in type 2 diabetic animals.METHODS:Type 2 diabetic rat models were induced by low-dose streptozotocin following high-fat diet feeding.A circular wound was made on the dorsum of the rats.The silicone splint was used to fix the wound.Skin thicknesses,microvessel densities,and the changes of collagen fibers and collagen levels of granulation tissues around the wound were tested by pathological examination.RESULTS AND CONCLUSION:Compared with normal group,after 72 hours of streptozotocin injection,fasting blood glucose,triglycerides and cholesterol of rats fed with high-fat diet were significantly increased(P < 0.05-0.01);after 1,3,5,7 and 14 days of injury,the skin thickness,microvascular density,collagen fibers and collagen content in the diabetes group were significantly reduced compared with the normal group(P < 0.01).The results indicated that high-fat diet combined with streptozotocin is simple and effective in establishment of type 2 diabetic models in rats,and wound healing of diabetic rats is more difficult than that of normal rats.