杜仲水/醇提取物促进兔脂肪基质干细胞增殖及成骨分化

背景:传统中药杜仲可以促进脂肪间充质干细胞成骨分化。目的:探讨杜仲水/醇提取物对脂肪基质干细胞的增殖作用和成骨分化影响。方法:取兔脂肪组织体外培养脂肪基质干细胞,传代3次后进行细胞增殖及成骨诱导分化。分为诱导培养基中分别含有杜仲水/醇提取物0.2,0.3,0.4,0.5g/L4个稀释度,对照组加入等量磷酸盐缓冲液。成骨诱导6d后进行各组细胞增殖活力的检测和碱性磷酸酶的测定,14d进行茜素红染色及钙化结节计数。结果与结论:杜仲水/醇提取物对脂肪基质干细胞的增殖无明显影响,但均可明显刺激上调碱性磷酸酶的活性,相同稀释度下杜仲醇提取物的调节作用大于水提取物,且在0.4~0.5g/L稀释度下对钙化结节的形成状况和数量有显著促进作用(P<0.05)。说明杜仲水/醇提取物有促进脂肪基质干细胞向成骨细胞转化的作用,杜仲醇提取物作用优于杜仲水提取物。

Effects of eucommia water/alcohol extract on proliferation and osteogenic differentiation of rabbit adipose derived mesenchymal stem cells

BACKGROUND：Conventional Chinese medicine eucommia can promote the osteogenic differentiation of adipose derived mesenchymal stem cells. OBJECTIVE：To investigate the effects of eucommia water/alcohol extract on the proliferation and osteogenic differentiation of adipose derived mesenchymal stem cells. METHODS：The rabbit adipose tissue was taken for in vitro culture of adipose derived mesenchymal stem cells. Passage 3 cells were used for study of cell proliferation ability and osteogenic differentiation. The experimental group cells were treated with induced medium containing 0.2, 0.3, 0.4, 0.5 g/L eucommia water/alcohol extract. The control group cells were treated with equal amounts of phosphate buffered saline. After osteogenic induction for 6 days, cell proliferation ability and alkaline phosphatase activity were determined. After osteogenic inducation for 14 days, alizarin red staining was performed and calcified nodules were counted.RESULTS AND CONCLUSION：Eucommia water/alcohol extract did not produce effects on the proliferation of adipose derived mesenchymal stem cells, but it significantly up-regulated the activity of alkaline phsphatase. Eucommia alcohol extract exhibited stronger regulatory effects than eucommia water extract at the same dilutions, and eucommia water/alcohol extract at 0.4-0.5 g/L dilutions significantly promoted the formation of calcified nodules （P 0.05）. These findings suggest that eucommia water/alcohol extract promotes the osteogenic differentiation of adipose derived mesenchymal stem cells, and eucommia alcohol extract exhibits better effects than eucommia water extract.