双能X射线骨密度仪不同测量方式检测大鼠骨密度的准确性

背景:双能X射线骨密度仪是诊断骨质疏松症的金标准,但采用其系统默认方式测量小动物骨密度误差很大。目的:观察双能X射线骨密度仪不同测量方式对大鼠骨密度测量准确度的影响。方法:应用双能X射线骨密度仪对六七月龄雌性SD大鼠进行全身扫描,分别采用自定义手动矩形方式、手动椭圆形方式与系统默认标准方式依次测量大鼠的全身、头部及脊柱部位的骨密度。结果与结论:手动椭圆形方式与系统默认方式测得的大鼠全身、头部和脊柱的骨密度差异无显著性意义(P>0.05),而手动矩形方式与系统默认标准方式间差异有显著性意义(P<0.01)。双能X射线骨密度仪应用手动椭圆形方式与系统默认标准方式对测量结果影响不大,但手动矩形测量方式误差较大。提示手动椭圆形方式可作为小动物骨密度测量后的分析方法之一。

Accuracy of body mineral density of rats with different measurement methods by dual energy X ray absorptometry

BACKGROUND:Dual energy X-ray absorptiometry is regarded as the gold standard for osteoporosis diagnosing,but its system default measurement method has large errors on the measurement of small animal bone density.OBJECTIVE:To study the effect of different measurement methods of dual energy X ray absorptiometry on the accuracy of bone mineral density measurement.METHODS:Whole body scan of 6-7 months female SD rats was performed by dual energy X ray absorptiometry.Custom manual rectangular pattern,custom manual oval way and system default standard way were used respectively to measure the bone mineral density of rat body,head and spine.RESULTS AND CONCLUSION:There was no significant difference of the body,head and spine bone mineral density measured by custom manual elliptic way and system default standard way(P > 0.05),while the bone mineral density measured by custom manual rectangular pattern and the system default standard methods was significantly different(P < 0.01).The manual elliptic way and the system default standard way of dual-energy X ray absorptiometry have little effect on the measurement results,but the custom manual rectangular pattern has large error.Manually elliptic way can be considered as one of the analysis method after small animal bone mineral density measurement.